| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRRM2 |
| Uniprot No | Q9UQ35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1666-2089aa |
| 氨基酸序列 | RTARRGSRSSPEPKTKSRTPPRRRSSRSSPELTRKARLSRRSRSASSSPETRSRTPPRHRRSPSVSSPEPAEKSRSSRRRRSASSPRTKTTSRRGRSPSPKPRGLQRSRSRSRREKTRTTRRRDRSGSSQSTSRRRQRSRSRSRVTRRRRGGSGYHSRSPARQESSRTSSRRRRGRSRTPPTSRKRSRSRTSPAPWKRSRSRASPATHRRSRSRTPLISRRRSRSRTSPVSRRRSRSRTSVTRRRSRSRASPVSRRRSRSRTPPVTRRRSRSRTPTTRRRSRSRTPPVTRRRSRSRTPPVTRRRSRSRTSPITRRRSRSRTSPVTRRRSRSRTSPVTRRRSRSRTSPVTRRRSRSRTPPAIRRRSRSRTPLLPRKRSRSRSPLAIRRRSRSRTPRTARGKRSLTRSPPAIRRRSASGSSSDRSR |
| 预测分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及SRRM2蛋白功能及其相关研究的文献概览：
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1. **文献名称**：*"SRRM2 organizes splicing condensates to regulate alternative splicing in neurons"*
**作者**：Takeuchi, A. et al.
**摘要**：研究发现SRRM2通过调控核内相分离形成的剪接体凝聚体，影响神经元中mRNA的可变剪接过程，揭示了其在神经发育中的关键作用。
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2. **文献名称**：*"The splicing factor SRRM2 regulates expression of ribosomal proteins to maintain cell homeostasis"*
**作者**：Siqueira, R.P. et al.
**摘要**：该文献指出SRRM2通过调控核糖体蛋白编码基因的剪接，参与维持细胞稳态，其缺失会导致核糖体生物合成异常和细胞周期停滞。
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3. **文献名称**：*"SRRM2 interacts with the C-terminal domain of RNA polymerase II and modulates transcriptional elongation"*
**作者**：Blazek, D. et al.
**摘要**：研究发现SRRM2直接结合RNA聚合酶II的C端结构域(CTD)，影响转录延伸过程，并揭示了其在转录-剪接偶联中的双重功能。
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**注**：上述文献为示例性内容，实际研究中建议通过PubMed或Web of Science检索最新文献(关键词：SRRM2. spliceosome, nuclear speckles)。真实文献可能更侧重SRRM2在剪接体组装、相分离或疾病(如癌症、神经退行性疾病)中的机制研究。
**Background of SRRM2 Recombinant Protein**
SRRM2 (Serine/Arg-rich Repetitive Matrix 2), also known as SRm300. is a nuclear scaffold protein integral to spliceosome assembly and pre-mRNA splicing. It belongs to the SR protein family, characterized by serine/arginine-rich domains critical for protein-protein and protein-RNA interactions. SRRM2 is a large (~300 kDa) multidomain protein containing multiple disordered regions, enabling dynamic interactions within nuclear speckles—subnuclear compartments enriched in splicing factors.
Functionally, SRRM2 acts as a scaffolding hub, coordinating the spatial organization of spliceosomal components and other RNA-processing factors. It interacts with key splicing regulators like SRSF1 and components of the exon junction complex, facilitating spliceosome assembly and coupling splicing with downstream mRNA export and translation. Studies also implicate SRRM2 in maintaining nuclear speckle integrity and mediating transcriptional regulation through chromatin interactions.
Recombinant SRRM2 proteins are engineered to study its molecular interactions, post-translational modifications (e.g., phosphorylation), and roles in diseases. Dysregulation of SRRM2 is linked to cancer, neurodegenerative disorders, and developmental defects, likely due to disrupted RNA processing. Recombinant variants enable in vitro assays, structural studies, and screening for therapeutic targets.
In summary, SRRM2 is a versatile scaffold protein central to RNA metabolism, with recombinant tools advancing mechanistic insights into its functions and disease associations.
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