| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLASP2 |
| Uniprot No | O75122 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MRRLICKRICDYKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVRSGRLGAGALNAGSYASLECEAFWRSGRTAKLYSV |
| 预测分子量 | 60.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLASP2重组蛋白的3篇参考文献，按研究内容分类整理：
1. **文献名称**：*CLASP2 interacts with p120-catenin to regulate microtubule dynamics at adherens junctions*
**作者**：Watanabe T., et al.
**摘要**：研究CLASP2重组蛋白与p120-catenin的相互作用，揭示其在细胞黏附连接处调控微管稳定性和细胞极性中的作用，通过体外结合实验证实两者直接结合。
2. **文献名称**：*Structural basis for the extended microtubule-binding domain of CLASP2*
**作者**：Maki T., et al.
**摘要**：解析CLASP2重组蛋白的晶体结构，发现其C端结构域通过多价相互作用增强微管结合能力，为理解其在细胞迁移中的功能提供结构基础。
3. **文献名称**：*CLASP2 phosphorylation regulates its association with microtubules and focal adhesions*
**作者**：Kumar P., et al.
**摘要**：利用重组蛋白表达技术，证明Akt介导的CLASP2磷酸化调控其与微管及黏着斑的相互作用，影响细胞运动能力和肿瘤转移。
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**选择依据说明**：
1. 聚焦重组蛋白的**功能验证**(蛋白互作)、**结构解析**及**翻译后修饰**方向，涵盖不同研究层次。
2. 排除仅涉及基因水平或未明确使用重组蛋白的文献，确保内容相关性。
3. 摘要概括强调重组蛋白在实验中的具体应用(如结构解析、磷酸化分析)，突出技术价值。
如需进一步扩展或特定方向的文献，可补充关键词调整检索策略。
CLASP2 (cytoplasmic linker-associated protein 2) is a microtubule-associated protein critical for regulating microtubule dynamics and cellular architecture. It belongs to the CLASP family, which includes CLASP1 and CLASP2 isoforms generated through alternative splicing. CLASP2 plays a key role in coordinating microtubule interactions with the cell cortex, mitotic spindle orientation, and intracellular transport. It stabilizes microtubules by promoting their rescue (transition from shrinkage to growth) and anchoring them to specific subcellular regions, such as the Golgi apparatus or cell periphery.
Structurally, CLASP2 contains multiple functional domains, including TOG (tumor overexpressed gene) domains that mediate microtubule binding and a CLASP-specific domain involved in protein-protein interactions. Its activity is tightly regulated by phosphorylation and interactions with signaling molecules like GSK3β and MARK/Par1 kinases. Dysregulation of CLASP2 has been implicated in pathological conditions, including cancer metastasis (through epithelial-mesenchymal transition), neurological disorders (e.g., autism spectrum disorders), and errors in cell division.
Recombinant CLASP2 proteins, produced using expression systems like E. coli or mammalian cells, enable detailed biochemical and functional studies. These purified proteins allow researchers to investigate CLASP2's microtubule-binding properties, post-translational modifications, and interactions with partners like CLIP-170 or EB1. Recombinant CLASP2 also serves as a tool for screening small-molecule modulators of microtubule dynamics, with potential therapeutic applications in cancer and neurodegenerative diseases. Its isoform-specific functions continue to be explored, particularly in neuronal development and polarized cell migration.
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