| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Mcu |
| Uniprot No | Q3UMR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 50-350aa |
| 氨基酸序列 | AHQRPASWQSVGAAYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVRPPKRDLLSHEDAATLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLKQQLAPLEKVRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYAYFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGEKE |
| 预测分子量 | 39,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MCU(线粒体钙离子单向转运蛋白)重组蛋白的关键文献摘要：
1. **"Integrative Genomics Identifies MCU as an Essential Component of the Mitochondrial Calcium Uniporter"**
- **作者**: Baughman, J.M. 等 (2011. *Nature*)
- **摘要**: 通过全基因组RNA干扰筛选，首次鉴定MCU为线粒体钙离子摄取的核心通道蛋白，并证明其缺失会显著抑制线粒体钙信号及细胞凋亡。
2. **"MICU1 Controls Both the Threshold and Cooperative Activation of the Mitochondrial Ca²⁺ Uniporter"**
- **作者**: Csordás, G. 等 (2013. *Cell*)
- **摘要**: 揭示MICU1作为MCU的调控亚基，通过感知胞质钙浓度动态调节MCU通道活性，防止线粒体钙超载及氧化损伤。
3. **"Structure and Function of the Mitochondrial Calcium Uniporter Complex"**
- **作者**: Fan, C. 等 (2020. *Nature*)
- **摘要**: 利用冷冻电镜解析MCU复合体的高分辨率结构，阐明其钙选择性渗透的分子机制及EMRE亚基对通道组装的关键作用。
4. **"Reconstitution of the Mitochondrial Calcium Uniporter in Yeast"**
- **作者**: Vais, H. 等 (2016. *Cell Reports*)
- **摘要**: 在酵母中异源表达MCU及其调控蛋白，成功重建功能性钙摄取系统，验证MCU单独足以形成钙通道的基础活性。
这些研究涵盖了MCU的发现、调控机制、结构解析及功能重建，为理解线粒体钙信号提供了重要基础。
Here’s a concise overview of MCU (mitochondrial calcium uniporter) recombinant protein:
The mitochondrial calcium uniporter (MCU) is a highly selective calcium channel located in the inner mitochondrial membrane, playing a pivotal role in cellular calcium signaling and energy metabolism. Its discovery in 2011 marked a breakthrough in understanding mitochondrial calcium regulation. MCU forms a oligomeric complex with regulatory subunits (e.g., MICU1. EMRE) that fine-tune calcium uptake under varying physiological conditions.
Recombinant MCU proteins are engineered through molecular cloning techniques, typically expressed in heterologous systems like *E. coli* or mammalian cell cultures. These recombinant versions enable structural and functional studies that were challenging with native proteins due to low abundance. Key applications include:
- Elucidating pore architecture using cryo-EM
- Characterizing calcium flux mechanisms
- Screening pharmacological modulators
- Investigating pathological correlations (e.g., neurodegeneration, cardiac dysfunction)
Recent structural studies using recombinant MCU revealed its pentameric assembly with a conserved DIME pore motif critical for ion selectivity. CRISPR-based screening has further uncovered tissue-specific isoforms (MCUb) that regulate channel activity. However, challenges persist in reconstituting full regulatory complexes *in vitro* and understanding post-translational modifications affecting channel gating.
The development of MCU recombinant proteins has accelerated therapeutic research targeting mitochondrial calcium dysregulation in conditions ranging from ischemic injury to metabolic disorders. Current efforts focus on designing isoform-specific inhibitors and leveraging recombinant systems to decode calcium-dependent apoptosis mechanisms.
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