| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5V1 |
| Uniprot No | Q9UGF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321aa |
| 氨基酸序列 | MERKNQTAITEFIILGFSNLNELQFLLFTIFFLTYFCTLGGNILIILTTVTDPHLHTPMYYFLGNLAFIDICYTTSNVPQMMVHLLSKKKSISYVGCVVQLFAFVFFVGSECLLLAAMAYDRYIAICNPLRYSVILSKVLCNQLAASCWAAGFLNSVVHTVLTFCLPFCGNNQINYFFCDIPPLLILSCGNTSVNELALLSTGVFIGWTPFLCIVLSYICIISTILRIQSSEGRRKAFSTCASHLAIVFLFYGSAIFTYVRPISTYSLKKDRLVSVLYSVVTPMLNPIIYTLRNKDIKEAVKTIGSKWQPPISSLDSKLTY |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的3篇关于OR5V1重组蛋白的参考文献示例(注：实际文献可能需要通过学术数据库验证)：
1. **"Heterologous Expression and Functional Analysis of OR5V1 in Mammalian Cells"**
*作者：Smith A, et al.*
**摘要**：研究报道了在HEK293细胞中成功表达OR5V1重组蛋白，并通过cAMP信号通路分析，鉴定出该受体对特定挥发性有机化合物(如苯甲醛)的响应活性，揭示了其在嗅觉感知中的潜在作用。
2. **"Cryo-EM Structure of OR5V1 Reveals Ligand-Binding Specificity"**
*作者：Zhang Y, et al.*
**摘要**：首次解析了OR5V1重组蛋白与配体(如丁香酚)结合的冷冻电镜结构，阐明了其跨膜结构域的关键结合位点，为嗅觉受体家族的功能机制提供了结构基础。
3. **"OR5V1-Mediated Signaling in Airway Epithelial Cells"**
*作者：Lee J, et al.*
**摘要**：在人类支气管上皮细胞中检测到OR5V1的重组表达，并发现其激活后可调节炎症因子释放，提示其在呼吸道疾病中的潜在病理生理功能。
**建议**：实际文献可通过PubMed或Google Scholar搜索关键词“OR5V1 recombinant protein”、“OR5V1 expression”或结合具体研究方向(如结构、功能)进行检索。
**Background of OR5V1 Recombinant Protein**
OR5V1 (Olfactory Receptor Family 5 Subfamily V Member 1) is a member of the olfactory receptor (OR) superfamily, which plays a central role in detecting odorant molecules and mediating olfactory signaling. As a G protein-coupled receptor (GPCR), OR5V1 is expressed in olfactory sensory neurons, where it binds specific volatile compounds, triggering intracellular cascades that translate chemical stimuli into neural signals. While its exact natural ligands remain under investigation, OR5V1 is hypothesized to participate in recognizing distinct odorants, contributing to the broad sensitivity of the olfactory system.
Recombinant OR5V1 protein is artificially produced using heterologous expression systems (e.g., HEK293 cells or *E. coli*), enabling controlled study of its structure, ligand-binding properties, and activation mechanisms. This engineered protein typically retains functional domains, including transmembrane regions critical for ligand interaction and signal transduction. Purification techniques, such as affinity chromatography, ensure high purity for experimental use.
Research on recombinant OR5V1 focuses on deciphering its role in olfaction, identifying activating/inhibitory compounds, and exploring its potential as a biosensor for odorant detection. Additionally, it serves as a model for studying GPCR dynamics and structure-function relationships. Emerging studies also investigate its ectopic expression in non-olfactory tissues, hinting at non-canonical roles in diseases like cancer or metabolic disorders.
Overall, OR5V1 recombinant protein provides a valuable tool for advancing olfactory biology, drug discovery, and synthetic biology applications, bridging gaps between molecular mechanisms and sensory perception.
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