| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Atp5j |
| Uniprot No | P18859 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-108aa |
| 氨基酸序列 | NKELDPIQKLFVDKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEVIEKPQA |
| 预测分子量 | 36.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5J重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：Recombinant expression and functional characterization of the ATP synthase subunit e in Saccharomyces cerevisiae
**作者**：Smith A, et al.
**摘要**：本研究在大肠杆菌中重组表达了ATP5J(ATP synthase subunit e)，通过亲和层析纯化并验证其与线粒体ATP合酶复合物的结合能力，证实其在质子通道功能中的作用。
2. **文献名称**：Structural insights into the ATP synthase rotor ring assembly using recombinant ATP5J protein
**作者**：Chen L, et al.
**摘要**：利用重组ATP5J蛋白进行冷冻电镜分析，揭示了该亚基在ATP合酶转子环组装中的关键结构域，并发现其突变会导致线粒体能量代谢异常。
3. **文献名称**：ATP5J2 recombinant protein enhances cellular oxidative phosphorylation in metabolic disease models
**作者**：Wang Y, et al.
**摘要**：通过哺乳动物细胞系统表达ATP5J2重组蛋白，证明其可恢复疾病模型中ATP合酶活性，改善细胞能量代谢障碍，为治疗线粒体相关疾病提供潜在策略。
注：上述文献信息为模拟示例，实际研究中建议通过PubMed或Web of Science以关键词"ATP5J recombinant"检索最新文献。
**Background of ATP5J Recombinant Protein**
ATP5J, also known as ATP synthase membrane subunit F6. is a critical component of the mitochondrial ATP synthase complex (Complex V), which drives the synthesis of adenosine triphosphate (ATP) during oxidative phosphorylation. This enzyme is essential for cellular energy production, coupling the proton gradient across the mitochondrial inner membrane with ATP generation. The ATP5J subunit is encoded by the nuclear *ATP5PF* gene in humans and forms part of the F0 proton-translocating domain of ATP synthase. It plays a structural and functional role in stabilizing the F0 sector and facilitating proton channeling, which is necessary for the rotational mechanism of ATP synthesis.
Recombinant ATP5J protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce a purified form of the subunit for functional and structural studies. Its recombinant expression enables researchers to investigate ATP synthase assembly, proton transport mechanisms, and mutations linked to mitochondrial disorders, such as Leigh syndrome or neuropathy. Additionally, ATP5J recombinant protein serves as a tool for studying cellular energy metabolism dysregulation in diseases like cancer, neurodegenerative conditions, and metabolic syndromes.
The protein is typically purified via affinity chromatography and validated through techniques like SDS-PAGE, Western blotting, or mass spectrometry to ensure homogeneity and bioactivity. By providing a controlled and scalable source of ATP5J, recombinant technology supports drug discovery efforts targeting mitochondrial dysfunction and advances the development of therapies for energy-related pathologies.
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