| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COA1 |
| Uniprot No | Q9GZY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-146aa |
| 氨基酸序列 | QKFHSRALYYKLAVEQLQSHPEAQEALGPPLNIHYLKLIDRENFVDIVDAKLKIPVSGSKSEGLLYVHSSRGGPFQRWHLDEVFLELKDGQQIPVFKLSGENGDEVKKE |
| 预测分子量 | 39.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与COA1重组蛋白相关的模拟参考文献(基于领域知识整合，非真实文献)：
1. **文献名称**: "COA1 is a mitochondrial complex IV assembly factor critical for electron transfer activity"
**作者**: Pierrel, F. et al.
**摘要**: 研究报道了人源COA1蛋白的重组表达与功能，发现其通过与SURF1相互作用参与细胞色素c氧化酶(COX)的组装，RNA干扰敲低COA1导致COX活性下降及线粒体呼吸缺陷。
2. **文献名称**: "Recombinant COA1 protein restores mitochondrial function in yeast coa1Δ mutants"
**作者**: Mick, D.U. et al.
**摘要**: 通过在大肠杆菌中重组表达酵母COA1同源蛋白，验证其可回补酵母coa1Δ突变体的OXPHOS缺陷，证实COA1在跨物种间保守的复合物IV组装功能。
3. **文献名称**: "Structural insights into COA1's role in mitochondrial translation regulation"
**作者**: Ghezzi, D. et al.
**摘要**: 利用重组COA1蛋白进行晶体结构解析，揭示其N端结构域与线粒体核糖体的结合机制，提出COA1通过调控MT-CO1 mRNA翻译参与COX亚基稳态平衡。
注：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索关键词"COA1 recombinant protein"或"COA1 complex IV assembly"获取。
**Background of COA1 Recombinant Protein**
COA1 (Cytochrome c Oxidase Assembly Factor 1), also known as MITRAC15. is a nuclear-encoded mitochondrial protein critical for the assembly and stability of cytochrome c oxidase (Complex IV) in the electron transport chain (ETC). This protein plays a vital role in mitochondrial oxidative phosphorylation (OXPHOS) by facilitating the incorporation of subunits into the nascent Complex IV structure. COA1 interacts with mitochondrial translation products and assembly factors, ensuring proper maturation of the enzyme complex.
Structurally, COA1 is anchored to the mitochondrial inner membrane and contains conserved domains that mediate protein-protein interactions. Dysregulation of COA1 has been linked to mitochondrial disorders, including Leigh syndrome and other OXPHOS deficiencies, underscoring its importance in cellular energy production.
Recombinant COA1 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling studies on its molecular functions, interactions, and role in disease. Researchers utilize this protein to investigate mitochondrial biogenesis, ETC dysfunction, and metabolic reprogramming in cancer or neurodegenerative diseases. Additionally, it serves as a tool for screening therapeutic agents targeting mitochondrial disorders.
Recent studies highlight COA1's potential as a biomarker for mitochondrial health and its involvement in stress responses, making it a focal point for understanding cellular energy regulation and developing precision medicine strategies.
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