| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATPAF2 |
| Uniprot No | Q8N5M1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 41-289aa |
| 氨基酸序列 | APPTERKRFYQNVSITQGEGGFEINLDHRKLKTPQAKLFTVPSEALAIAVATEWDSQQDTIKYYTMHLTTLCNTSLDNPTQRNKDQLIRAAVKFLDTDTICYRVEEPETLVELQRNEWDPIIEWAEKRYGVEISSSTSIMGPSIPAKTREVLVSHLASYNTWALQGIEFVAAQLKSMVLTLGLIDLRLTVEQAVLLSRLEEEYQIQKWGNIEWAHDYELQELRARTAAGTLFIHLCSESTTVKHKLLKE |
| 预测分子量 | 32.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATPAF2重组蛋白的参考文献示例(内容为模拟概括，非真实文献)：
1. **文献名称**: "Functional characterization of ATPAF2 in mitochondrial ATP synthase assembly"
**作者**: Smith J, et al.
**摘要**: 本研究利用重组ATPAF2蛋白，揭示了其在哺乳动物线粒体ATP合酶复合体组装中的关键作用，通过体外实验证明ATPAF2与ATP合酶亚基的相互作用，并发现其缺陷导致细胞能量代谢异常。
2. **文献名称**: "Recombinant ATPAF2 expression in E. coli and its role in yeast mitochondrial function"
**作者**: Lee S, et al.
**摘要**: 报道了通过大肠杆菌系统高效表达重组ATPAF2蛋白的方法，并利用酵母模型验证其功能，证实ATPAF2缺失导致线粒体膜电位下降和ATP合成减少。
3. **文献名称**: "ATPAF2 mutations and structural analysis of recombinant protein in neurological disorders"
**作者**: Garcia R, et al.
**摘要**: 通过重组ATPAF2蛋白的晶体结构解析，结合患者突变体分析，揭示特定氨基酸位点变异如何破坏ATP合酶组装，进而导致神经退行性疾病的发生机制。
4. **文献名称**: "Proteomic interaction network of ATPAF2 recombinant protein in cardiac cells"
**作者**: Chen L, et al.
**摘要**: 利用重组ATPAF2蛋白进行心脏细胞蛋白质互作组学分析，发现其与多种线粒体伴侣蛋白协同调控ATP合酶稳定性，为心肌病治疗提供潜在靶点。
注：以上为模拟文献，实际研究中建议通过PubMed、Web of Science等平台以关键词“ATPAF2 recombinant protein”或“ATPAF2 mitochondrial assembly”检索真实文献。
**Background of ATPAF2 Recombinant Protein**
ATPAF2 (ATP Synthase Mitochondrial F1 Complex Assembly Factor 2) is a nuclear-encoded protein crucial for the assembly and functional integrity of mitochondrial ATP synthase (Complex V), a key enzyme in oxidative phosphorylation. This enzyme catalyzes ATP production, essential for cellular energy homeostasis. ATPAF2 specifically facilitates the proper assembly of the F1 catalytic core (α3β3γ subcomplex) within the mitochondrial matrix, ensuring structural stability and enzymatic activity of ATP synthase.
Dysregulation of ATPAF2 has been linked to mitochondrial disorders, often presenting as neuromuscular or metabolic diseases due to impaired energy production. Studying ATPAF2 helps elucidate molecular mechanisms underlying mitochondrial dysfunction and associated pathologies.
Recombinant ATPAF2 protein is generated via heterologous expression systems (e.g., *E. coli* or mammalian cells*) by cloning the ATPAF2 gene into expression vectors, followed by purification using affinity chromatography. This engineered protein retains biological activity, enabling *in vitro* studies such as protein-protein interaction assays, enzyme reconstitution experiments, and structural analyses. Researchers utilize recombinant ATPAF2 to investigate its role in ATP synthase biogenesis, screen for potential therapeutic compounds targeting mitochondrial deficiencies, or model mutations identified in genetic disorders.
Overall, ATPAF2 recombinant protein serves as a vital tool for advancing our understanding of mitochondrial biology and developing strategies to treat energy metabolism-related diseases.
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