| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEACAM4 |
| Uniprot No | O75871 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-155aa |
| 氨基酸序列 | FTIEALPSSAAEGKDVLLLACNISETIQAYYWHKGKTAEGSPLIAGYITDIQANIPGAAYSGRETVYPNGSLLFQNITLEDAGSYTLRTINASYDSDQATGQLHVHQNNVPGLPVGAVAG |
| 预测分子量 | 14.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEACAM4重组蛋白的模拟参考文献示例(注：以下文献为假设性示例，实际文献需通过学术数据库核实)：
---
1. **标题**: *Cloning and Functional Analysis of Recombinant CEACAM4 in Gastric Cancer*
**作者**: Tanaka K, et al.
**摘要**: 本研究成功克隆并表达了人源CEACAM4重组蛋白，发现其在胃癌细胞中高表达，并通过体外实验证实其促进肿瘤细胞迁移的作用，提示其作为潜在治疗靶点。
2. **标题**: *Expression and Purification of CEACAM4 Recombinant Protein for Immunoassay Development*
**作者**: Müller S, et al.
**摘要**: 报道了一种高效表达和纯化CEACAM4重组蛋白的方法，优化了哺乳动物细胞表达系统，并验证其在ELISA检测中的特异性，为临床诊断工具开发奠定基础。
3. **标题**: *CEACAM4 Recombinant Protein Modulates Immune Checkpoint Signaling in Melanoma*
**作者**: Chen H, et al.
**摘要**: 通过重组CEACAM4蛋白研究其与PD-L1的相互作用，发现其可抑制T细胞活性，揭示了CEACAM4在肿瘤免疫逃逸中的潜在机制。
4. **标题**: *Structural Insights into CEACAM4 Recombinant Protein via Cryo-EM*
**作者**: Lee J, et al.
**摘要**: 利用冷冻电镜技术解析CEACAM4重组蛋白的高分辨率结构，发现其独特的胞外域构象，为设计靶向药物提供结构基础。
---
**建议**：如需真实文献，请访问PubMed或Google Scholar，使用关键词“CEACAM4 recombinant protein”、“CEACAM4 expression”或结合具体研究领域(如癌症、免疫)进行检索。
CEACAM4 (carcinoembryonic antigen-related cell adhesion molecule 4) is a member of the CEACAM family, a group of glycoproteins within the immunoglobulin superfamily involved in intercellular adhesion, immune regulation, and signaling. Primarily expressed in granulocytes and some epithelial tissues, CEACAM4 is implicated in modulating immune responses, particularly in inflammation and pathogen recognition. Its structure includes an extracellular N-terminal domain, transmembrane region, and cytoplasmic tail, which may interact with intracellular signaling molecules. Though less studied than other CEACAMs (e.g., CEACAM5/CEA or CEACAM6), CEACAM4 has drawn interest for its potential role in cancer biology and immune evasion, with altered expression observed in certain malignancies.
Recombinant CEACAM4 protein is produced using genetic engineering techniques, often in bacterial (e.g., E. coli) or mammalian expression systems, to ensure proper folding and post-translational modifications. This engineered protein retains key functional domains, enabling researchers to study its interactions with ligands, receptors, or pathogens in vitro. Applications include elucidating CEACAM4’s role in neutrophil activation, microbial adhesion (e.g., binding to bacterial proteins), and tumor microenvironments. It also serves as an antigen for antibody development or diagnostic assays. By providing a controlled, pure form of the protein, recombinant CEACAM4 facilitates mechanistic studies and therapeutic exploration, such as targeting CEACAM4-associated pathways in inflammatory diseases or cancer immunotherapy. Ongoing research aims to clarify its dual roles in host defense and pathological processes.
×
