| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cd68 |
| Uniprot No | P34810 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-319aa |
| 氨基酸序列 | MGSSHPLHHHSSGLVPRGSHMGSHMNDCPHKKSATLLPSFTVTPTVTEST GTTSHRTTKSHKTTTHRTTTTGTTSHGPTTATHNPTTTSHGNVTVHPTSN STATSQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPEPPPPS PSPSPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVL NPNKTKVQGSCEGAHPHLLLSFPYGHLSFGFMQDLQQKVVYLSYMAVEYN VSFPHAAQWTFSAQNASLRDLQAPLGQSFSCSNSSIILSPAVHLDLLSLR LQAAQLPHTGVFGQSFSCPSDRS |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD68重组蛋白的3篇代表性文献的简要信息(注：以下为模拟内容，实际文献可能需要通过数据库查询确认)：
1. **《CD68 recombinant protein expression and macrophage-specific localization》**
- 作者：Smith A, et al.
- 摘要：研究通过原核系统表达CD68重组蛋白，验证其与巨噬细胞表面特异性结合的能力，证实其作为单核/巨噬细胞谱系标志物的应用价值。
2. **《Characterization of recombinant human CD68 as a diagnostic marker for inflammatory diseases》**
- 作者：Li X, et al.
- 摘要：构建人源CD68重组蛋白，通过免疫组化分析其在动脉粥样硬化等炎症病变组织中的高表达，提出其作为炎症诊断工具的潜力。
3. **《Structural and functional analysis of CD68 protein domains》**
- 作者：Wang Y, et al.
- 摘要：利用重组CD68蛋白解析其LAMP家族结构域的功能，发现其与溶酶体膜结合的机制，为研究巨噬细胞分化提供分子基础。
如需具体文献，建议通过PubMed或Web of Science以关键词“CD68 recombinant protein”检索近年研究。
**Background of CD68 Recombinant Protein**
CD68. a glycoprotein encoded by the *CD68* gene in humans, is a member of the lysosome-associated membrane protein (LAMP) family. It is predominantly expressed on the surface of monocytes, macrophages, and dendritic cells, serving as a well-established marker for identifying these immune cell types. Structurally, CD68 contains a highly glycosylated luminal domain and a short cytoplasmic tail, which facilitates its localization to lysosomes and endosomes. Its primary biological role involves participating in intracellular trafficking, phagocytosis, and antigen processing, contributing to innate immune responses and tissue homeostasis.
Recombinant CD68 protein is engineered using molecular cloning techniques, often expressed in mammalian or bacterial systems (e.g., HEK293 or *E. coli*), followed by purification via affinity chromatography. This engineered protein retains key functional domains, enabling researchers to study CD68-mediated pathways in vitro or in vivo. Applications include investigating macrophage activation, inflammatory diseases (e.g., atherosclerosis, rheumatoid arthritis), and tumor-associated macrophage (TAM) behavior in cancer microenvironments. Additionally, recombinant CD68 serves as a critical tool for developing antibodies, validating diagnostic assays, and screening therapeutic agents targeting macrophage-related pathologies.
Studies leveraging recombinant CD68 have clarified its interactions with scavenger receptors and toll-like receptors (TLRs), highlighting its role in lipid metabolism and pathogen recognition. Despite its utility, challenges remain in mimicking native post-translational modifications (e.g., glycosylation) in certain expression systems, necessitating careful experimental design. Overall, CD68 recombinant protein remains a vital resource for dissecting immune mechanisms and advancing therapeutic strategies in inflammatory and oncological research.
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