| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCT6A |
| Uniprot No | P40227 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-531aa |
| 氨基酸序列 | AAVKTLNPKAEVARAQAALAVNISAARGLQDVLRTNLGPKGTMKMLVSGAGDIKLTKDGNVLLHEMQIQHPTASLIAKVATAQDDITGDGTTSNVLIIGELLKQADLYISEGLHPRIITEGFEAAKEKALQFLEEVKVSREMDRETLIDVARTSLRTKVHAELADVLTEAVVDSILAIKKQDEPIDLFMIEIMEMKHKSETDTSLIRGLVLDHGARHPDMKKRVEDAYILTCNVSLEYEKTEVNSGFFYKSAEEREKLVKAERKFIEDRVKKIIELKRKVCGDSDKGFVVINQKGIDPFSLDALSKEGIVALRRAKRRNMERLTLACGGVALNSFDDLSPDCLGHAGLVYEYTLGEEKFTFIEKCNNPRSVTLLIKGPNKHTLTQIKDAVRDGLRAVKNAIDDGCVVPGAGAVEVAMAEALIKHKPSVKGRAQLGVQAFADALLIIPKVLAQNSGFDLQETLVKIQAEHSESGQLVGVDLNTGEPMVAAEVGVWDNYCVKKQLLHSCTVIATNILLVDEIMRAGMSSLKG |
| 预测分子量 | 61.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCT6A重组蛋白的3篇参考文献示例(文献名称和作者为虚构，仅供示例参考)：
1. **文献名称**：*Expression and Functional Analysis of Recombinant CCT6A in Cancer Cell Proliferation*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌系统表达重组CCT6A蛋白，并验证其在肺癌细胞中的促增殖作用，表明CCT6A可能通过调控细胞周期相关蛋白促进肿瘤生长。
2. **文献名称**：*Structural Insights into CCT6A Subunit in the Chaperonin Complex*
**作者**：Zhang L, et al.
**摘要**：通过冷冻电镜解析包含重组CCT6A的CCT复合体结构，揭示其在底物识别和ATP水解中的关键作用，为理解伴侣蛋白机制提供结构基础。
3. **文献名称**：*CCT6A Recombinant Protein Interacts with Tau in Neurodegenerative Models*
**作者**：Johnson R, et al.
**摘要**：在体外实验中，纯化的重组CCT6A蛋白与Tau蛋白直接结合，可能通过减少异常聚集延缓阿尔茨海默病模型中的神经退行性病变。
(注：以上文献为示例，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。)
**Background of CCT6A Recombinant Protein**
The **CCT6A** (Chaperonin Containing TCP-1 subunit 6A) protein is a critical component of the **eukaryotic chaperonin complex CCT** (also called TRiC), which facilitates ATP-dependent folding of newly synthesized polypeptides, particularly cytoskeletal proteins like actin and tubulin. This multi-subunit complex plays a vital role in maintaining proteostasis, ensuring proper protein conformation, and preventing aggregation.
CCT6A, encoded by the *CCT6A* gene in humans, is one of eight paralogous subunits (CCT1-8) that form the double-ring structure of the CCT complex. Each subunit exhibits unique substrate-binding specificity, with CCT6A implicated in interactions critical for folding actin and other client proteins. Dysregulation of CCT6A has been linked to diseases such as cancer, neurodegenerative disorders, and developmental defects, highlighting its importance in cellular health.
**Recombinant CCT6A protein** is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce purified, functional CCT6A for research. This allows scientists to study its structural properties, substrate-binding mechanisms, and role in chaperone-assisted folding *in vitro*. Recombinant versions are also used to investigate disease-related mutations, screen for therapeutic compounds targeting protein misfolding, or develop biomarkers.
The production of CCT6A recombinant protein often involves tagging (e.g., His-tag) for simplified purification and detection. Its applications span structural biology (cryo-EM studies), drug discovery, and mechanistic studies of proteostasis networks. Ongoing research continues to uncover its interactions with oncogenic proteins or amyloidogenic aggregates, positioning CCT6A as a potential target for therapies addressing protein misfolding diseases.
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