| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CACNA2D1 |
| Uniprot No | P54289 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 577-717aa |
| 氨基酸序列 | KMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLKPDNFEESGYTFIAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFTNELVQ |
| 预测分子量 | 32.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CACNA2D1重组蛋白的3篇文献示例(内容为模拟概括)：
1. **文献名称**: *"Expression and functional characterization of recombinant CACNA2D1 in HEK293 cells"*
**作者**: Smith A, et al.
**摘要**: 研究报道了在HEK293细胞中重组表达CACNA2D1蛋白的方法，验证其与钙通道α1亚基的相互作用，并证明其增强钙电流的功能特性。
2. **文献名称**: *"Structural insights into the α2δ-1 subunit for voltage-gated calcium channel complex assembly"*
**作者**: Wang B, et al.
**摘要**: 通过冷冻电镜解析CACNA2D1重组蛋白的晶体结构，揭示了其与α1亚基结合的分子机制，为钙通道复合体的组装提供结构基础。
3. **文献名称**: *"CACNA2D1 as a target for neuropathic pain: Role of recombinant protein in gabapentin binding"*
**作者**: Li Y, et al.
**摘要**: 利用重组CACNA2D1蛋白验证了抗癫痫药物加巴喷丁(gabapentin)的结合位点，探讨其在神经病理性疼痛治疗中的作用机制。
(注：以上文献信息为示例，实际引用需根据具体研究检索PubMed、Web of Science等数据库。)
CACNA2D1. a gene encoding the α2δ-1 subunit of voltage-gated calcium channels (VGCCs), plays a critical role in regulating calcium ion flux across cell membranes. This subunit is one of four α2δ paralogs (α2δ-1 to -4) and functions as an auxiliary component of the Cavα1 pore-forming subunit, enhancing channel trafficking to the plasma membrane and modulating electrophysiological properties. The α2δ-1 protein is post-translationally cleaved into two disulfide-linked subunits: α2. an extracellular glycosylated protein, and δ, a transmembrane anchor. It interacts with extracellular matrix proteins and intracellular signaling molecules, influencing channel kinetics and synaptic transmission.
Recombinant CACNA2D1 protein is commonly expressed in heterologous systems (e.g., mammalian or insect cells) for structural and functional studies. Its production enables detailed investigation of calcium channel assembly, ligand-receptor interactions, and mechanisms underlying neurological disorders. Notably, α2δ-1 is a high-affinity target for gabapentinoid drugs (e.g., gabapentin, pregabalin), widely used to treat neuropathic pain and epilepsy. Structural studies of recombinant α2δ-1 have revealed binding sites for these therapeutics, informing drug design strategies.
Pathologically, CACNA2D1 dysregulation is implicated in neuropathic pain, epilepsy, cardiovascular diseases, and cancer progression. Overexpression in sensory neurons enhances presynaptic calcium currents, contributing to chronic pain states. Recombinant protein tools aid in characterizing disease-associated mutations and screening potential modulators. Recent cryo-EM structures of full-length α2δ-1 complexed with Cav subunits have advanced understanding of VGCC architecture, while engineered soluble variants facilitate crystallographic studies. Ongoing research leverages recombinant α2δ-1 to decode its non-canonical roles in synaptogenesis and cell adhesion beyond calcium channel regulation.
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