| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX7A2L |
| Uniprot No | O14548 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-114aa |
| 氨基酸序列 | MYYKFSGFTQKLAGAWASEAYSPQGLKPVVSTEAPPIIFATPTKLTSDSTVYDYAGKNKVPELQKFFQKADGVPVYLKRGLPDQMLYRTTMALTVGGTIYCLIALYMASQPKNK |
| 预测分子量 | 39.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COX7A2L重组蛋白的3篇代表性文献，涵盖其功能研究、结构分析及技术应用方向：
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1. **文献名称**：*COX7A2L is a mitochondrial complex IV assembly factor critical for supercomplex formation*
**作者**：Mourier, A., et al.
**摘要**：本研究通过重组表达COX7A2L蛋白，结合基因敲除模型，发现其作为线粒体复合物IV(COX)的组装因子，对呼吸链超复合体(如III₂-IV)的稳定性至关重要。实验表明重组COX7A2L能恢复缺陷细胞的呼吸功能，证实其在能量代谢中的调控作用。
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2. **文献名称**：*Structural insights into COX7A2L-mediated respiratory chain organization*
**作者**：Cogliati, S., et al.
**摘要**：作者利用重组COX7A2L蛋白进行体外结合实验和冷冻电镜分析，揭示了其与复合物III/IV的相互作用界面，并提出其通过构象变化调节超复合体动态组装的分子机制。研究为靶向COX7A2L的代谢疾病治疗提供了结构基础。
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3. **文献名称**：*Recombinant COX7A2L protein rescues mitochondrial dysfunction in a cellular model of Leigh syndrome*
**作者**：Varanita, T., et al.
**摘要**：该研究通过大肠杆菌系统表达并纯化重组人源COX7A2L蛋白，证明其可递送至线粒体缺陷细胞中，显著改善复合物IV活性及ATP合成能力，为线粒体脑肌病的蛋白替代疗法提供了实验依据。
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**备注**：上述文献为模拟示例，实际引用时建议通过PubMed或Web of Science以“COX7A2L recombinant protein”为关键词检索最新研究。
COX7A2L (Cytochrome c Oxidase Subunit 7A2-Like) is a nuclear-encoded mitochondrial protein implicated in the structural organization and functional regulation of the electron transport chain (ETC). It shares homology with COX7A2. a subunit of cytochrome c oxidase (Complex IV), but differs in tissue-specific expression and splice variants. COX7A2L is notably involved in the assembly of mitochondrial respiratory supercomplexes—dynamic associations of ETC complexes (I, III, and IV) that optimize electron transfer efficiency and reduce oxidative stress. Studies suggest its role as a "supercomplex assembly factor," though this remains debated due to species-specific differences; for example, murine COX7A2L is critical for supercomplex formation, whereas human orthologs show more variable effects.
Recombinant COX7A2L protein is engineered for in vitro studies to dissect its molecular interactions, structural contributions, and regulatory mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it often includes tags (e.g., His-tag) for purification. Research applications include binding assays to map interactions with ETC components, functional assays to assess respiratory efficiency, and structural studies (e.g., cryo-EM) to visualize supercomplex architecture. Its recombinant form has also been used to explore tissue-specific roles, particularly in energy-demanding organs like the liver, heart, and skeletal muscle.
Emerging evidence links COX7A2L dysfunction to metabolic disorders, cancer, and neurodegenerative diseases, positioning it as a potential therapeutic target. However, mechanistic insights remain incomplete, driving demand for recombinant tools to unravel its context-dependent roles in mitochondrial physiology.
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