| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSRP3 |
| Uniprot No | P50461 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194aa |
| 氨基酸序列 | MPNWGGGAKCGACEKTVYHAEEIQCNGRSFHKTCFHCMACRKALDSTTVAAHESEIYCKVCYGRRYGPKGIGYGQGAGCLSTDTGEHLGLQFQQSPKPARSVTTSNPSKFTAKFGESEKCPRCGKSVYAAEKVMGGGKPWHKTCFRCAICGKSLESTNVTDKDGELYCKVCYAKNFGPTGIGFGGLTQQVEKKE |
| 预测分子量 | 37.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSRP3重组蛋白的3篇代表性文献概览(注：文献信息为模拟示例，具体内容请核实真实数据库)：
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1. **文献名称**：*Recombinant CSRP3 modulates cardiac hypertrophy signaling in vitro*
**作者**：Knöll R, et al.
**摘要**：本研究通过大肠杆菌表达重组人CSRP3蛋白，验证其在心肌细胞中的功能。实验表明，重组CSRP3通过调控肌动蛋白细胞骨架重塑，抑制血管紧张素II诱导的心肌细胞肥大，提示其可能作为心力衰竭治疗的潜在靶点。
2. **文献名称**：*Structural characterization of the LIM domains of CSRP3 using NMR spectroscopy*
**作者**：Flick MJ, Buck CB.
**摘要**：利用核磁共振技术解析重组CSRP3蛋白的LIM结构域三维结构，揭示其与锌离子结合的分子机制，并发现该结构域与心肌α-辅肌动蛋白的结合界面，为理解其在肌肉收缩中的作用提供结构基础。
3. **文献名称**：*CSRP3 as a serum biomarker in dilated cardiomyopathy: Development of a recombinant protein-based ELISA*
**作者**：Geier C, et al.
**摘要**：通过真核系统表达带His标签的重组CSRP3蛋白，建立高灵敏度ELISA检测法。临床样本分析显示，血清CSRP3水平与扩张型心肌病严重程度显著相关，证实其作为无创诊断标志物的潜力。
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如需进一步研究，建议在PubMed或Web of Science中以关键词“recombinant CSRP3 protein”“MLP expression”或“CSRP3 purification”检索最新文献。
**Background of CSRP3 Recombinant Protein**
CSRP3 (Cysteine and Glycine-Rich Protein 3), also known as Muscle LIM Protein (MLP), is a member of the CSRP family characterized by its LIM domain—a zinc-binding structure critical for protein-protein interactions. Primarily expressed in cardiac and skeletal muscle tissues, CSRP3 plays a pivotal role in maintaining cytoskeletal organization, regulating muscle cell differentiation, and modulating mechanical stress responses. It interacts with structural proteins like α-actinin and telethonin, as well as transcription factors such as MyoD, linking cellular architecture to gene expression.
Mutations in the *CSRP3* gene are associated with human cardiomyopathies, including hypertrophic (HCM) and dilated (DCM) cardiomyopathy, highlighting its importance in cardiac function. Studies in animal models (e.g., *Csrp3* knockout mice) reveal disrupted sarcomere organization, impaired cardiac contractility, and progressive heart failure, further underscoring its role in maintaining myocardial integrity.
Recombinant CSRP3 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, structural interactions, and therapeutic potential. This engineered protein retains functional domains, enabling research into its mechanosensory functions, involvement in hypertrophy signaling pathways (e.g., calcineurin/NFAT), and crosstalk with other LIM proteins. Applications span *in vitro* assays, disease modeling, and drug screening for cardiac disorders. Additionally, recombinant CSRP3 serves as an antigen for antibody development, aiding diagnostic and mechanistic studies in cardiomyopathy research. Its versatility makes it a valuable tool for unraveling molecular mechanisms of muscle diseases and exploring targeted therapies.
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