| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRX |
| Uniprot No | O43186 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSAPRKQRRERTTFTRSQ LEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCRQQRQQ QKQQQQPPGGQAKARPAKRKAGTSPRPSTDVCPDPLGISDSYSPPLPGPS GSPTTAVATVSIWSPASESPLPEAQRAGLVASGPSLTSAPYAMTYAPASA FCSSPSAYGSPSSYFSGLDPYLSPMVPQLGGPALSPLSGPSVGPSLAQSP TSLSGQSYGAYSPVDSLEFKDPTGTWKFTYNPMDPLDYKDQSAWKFQIL |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRX重组蛋白的3篇代表性文献的简要整理：
1. **《Production and functional characterization of recombinant CRX homeobox protein》**
- 作者：Chen, Y. et al.
- 摘要：该研究报道了通过大肠杆菌系统高效表达并纯化人源CRX重组蛋白，验证了其与视网膜特异性基因启动子的结合能力，为后续功能研究提供工具蛋白。
2. **《Disease-associated mutations in CRX reveal functional defects through recombinant protein analysis》**
- 作者：Wang, L. et al.
- 摘要：通过构建多种CRX致病突变体的重组蛋白，发现部分突变导致DNA结合亲和力下降及转录激活功能受损，解释了相关视网膜退行性疾病的分子机制。
3. **《AAV-mediated CRX gene delivery restores photoreceptor function in a murine model》**
- 作者：Johnson, B. et al.
- 摘要：利用重组AAV载体递送CRX基因至CRX敲除小鼠视网膜，成功恢复视锥/视杆细胞的光反应特性，证明了CRX蛋白在基因治疗中的潜在应用价值。
注：以上文献信息为基于领域研究的模拟概括，实际引用时建议通过PubMed或Web of Science核对具体论文。
CRX (Cone-Rod Homeobox) recombinant protein is a genetically engineered protein derived from the CRX gene, which encodes a transcription factor critical for photoreceptor development and maintenance in the retina. Discovered in the 1990s, CRX is a member of the homeobox gene family and plays a pivotal role in regulating the expression of photoreceptor-specific genes, including those involved in phototransduction and outer segment structure. Mutations in CRX are linked to inherited retinal diseases such as Leber congenital amaurosis (LCA), cone-rod dystrophy, and retinitis pigmentosa, highlighting its importance in retinal function.
Recombinant CRX protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling large-scale purification for research and therapeutic applications. Its production involves cloning the CRX gene into expression vectors, followed by protein extraction, affinity chromatography, and quality validation. The recombinant protein retains the functional domains of native CRX, including its DNA-binding homeodomain and transcriptional activation regions, allowing it to interact with target gene promoters in experimental settings.
Researchers utilize CRX recombinant protein to study photoreceptor development, gene regulation networks, and disease mechanisms. It serves as a tool for in vitro binding assays, chromatin immunoprecipitation (ChIP), and functional studies in retinal organoids or animal models. Additionally, it supports drug discovery efforts aimed at modulating CRX activity or rescuing pathogenic mutations. Recent advances in gene therapy have also explored CRX as a potential target for restoring vision in retinal degenerative diseases. By providing a controlled, reproducible source of CRX, recombinant protein technology accelerates both basic research and translational applications in ophthalmology.
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