| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCTN3 |
| Uniprot No | O75935 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-176aa |
| 氨基酸序列 | AGLTDLQRLQARVEELERWVYGPGGARGSRKVADGLVKVQVALGNISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQAPWGVGVRDEAGSLVEDVGFAQFLSVLHFGPTGPVCGNH |
| 预测分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCTN3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Recombinant expression and functional analysis of the dynactin subunit DCTN3 in microtubule anchoring"*
**作者**：Schroer, T.A., et al.
**摘要**：本研究通过大肠杆菌系统重组表达了DCTN3蛋白，并分析其与动力蛋白复合体其他亚基(如DCTN1/DCTN2)的相互作用。结果表明，DCTN3是维持复合体稳定性的关键组分，并参与调控微管锚定及细胞内运输功能。
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2. **文献名称**：*"Structural insights into the dynactin complex: Cryo-EM of recombinant DCTN3-bound DCTN1"*
**作者**：King, S.J., & Carter, A.P.
**摘要**：利用冷冻电镜技术解析了重组表达的DCTN3与DCTN1复合物的三维结构，揭示了DCTN3通过α-螺旋结构域与DCTN1结合，为阐明动力蛋白在细胞分裂中的调控机制提供了结构基础。
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3. **文献名称**：*"Dynactin subunit DCTN3 modulates dynein processivity through recombinant co-expression assays"*
**作者**：Huang, J., et al.
**摘要**：通过在昆虫细胞中重组共表达DCTN3与动力蛋白重链，发现DCTN3显著增强动力蛋白的运动持续力(processivity)，并证明其缺失会导致囊泡运输缺陷，提示其在神经退行性疾病中的潜在作用。
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注：以上文献为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合具体研究方向补充关键词(如“dynactin complex”“DCTN3 purification”)进一步筛选。
**Background of DCTN3 Recombinant Protein**
DCTN3 (Dynactin Subunit 3), also known as p22. is a critical component of the dynactin complex, a multi-subunit protein assembly required for the cytoplasmic dynein-driven retrograde transport of organelles, vesicles, and macromolecules along microtubules. As part of the dynactin complex, DCTN3 interacts with other subunits (e.g., DCTN1/p150-glued, DCTN2/p50) to stabilize dynein binding to cargo and enhance motor processivity. This complex plays a vital role in mitosis, Golgi organization, neuronal trafficking, and autophagy, linking cellular transport defects to neurodegenerative diseases (e.g., ALS) and cancer.
Recombinant DCTN3 protein is engineered via molecular cloning, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. It is purified using affinity tags (e.g., His-tag, GST) and validated via SDS-PAGE, Western blot, or mass spectrometry. Researchers utilize recombinant DCTN3 to study dynactin assembly, dynein-dynactin interactions, and intracellular transport mechanisms. It also serves as an antigen for antibody production or a tool in drug discovery targeting transport-related disorders.
Dysregulation of DCTN3 has been implicated in impaired axonal transport and protein aggregation in Alzheimer’s and Parkinson’s diseases. Its recombinant form enables mechanistic insights into these pathologies, offering potential for therapeutic screening. Overall, DCTN3 recombinant protein is a pivotal resource for dissecting microtubule-based transport biology and its disease connections.
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