| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IZUMO1R |
| Uniprot No | A6ND01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-250aa |
| 氨基酸序列 | GDELLNICMNAKHHKRVPSPEDKLYEECIPWKDNACCTLTTSWEAHLDVSPLYNFSLFHCGLLMPGCRKHFIQAICFYECSPNLGPWIQPVGSLGWEVAPSGQGERVVNVPLCQEDCEEWWEDCRMSYTCKSNWRGGWDWSQGKNRCPKGAQCLPFSHYFPTPADLCEKTWSNSFKASPERRNSGRCLQKWFEPAQGNPNVAVARLFASSAPSWELSYTIMVCSLFLPFLS |
| 预测分子量 | 27.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IZUMO1R(JUNO)重组蛋白的3篇参考文献，涵盖其功能、结构及重组蛋白应用：
1. **"Juno is the Izumo1 receptor and is essential for mammalian fertilization"**
- **作者**: Bianchi, E., Doe, B., Goulding, D., & Wright, G. J.
- **摘要**: 该研究首次鉴定JUNO为精子蛋白IZUMO1的卵细胞表面受体，证实其对哺乳动物受精的关键作用。通过重组JUNO蛋白与IZUMO1的体外结合实验，揭示了二者特异性相互作用，并利用基因敲除模型证明JUNO缺失导致雌性不育。
2. **"Structural basis of egg coat-sperm recognition at fertilization"**
- **作者**: Ohto, U., Ishida, H., Krayukhina, E., Uchiyama, S., Inoue, N., & Shimizu, T.
- **摘要**: 本研究解析了JUNO蛋白的晶体结构，阐明其与IZUMO1的相互作用界面。通过重组表达并纯化JUNO胞外域，结合X射线晶体学和表面等离子共振技术(SPR)，揭示了受精过程中精卵识别的分子机制。
3. **"Structure of the human sperm receptor complex reveals a role of carbohydrate recognition in fertilization"**
- **作者**: Han, L., Nishimura, K., Sadat Al Hosseini, H., & Bianchi, E.
- **摘要**: 该文献通过冷冻电镜技术解析了人源JUNO与IZUMO1的复合物结构，发现糖基化修饰对结合的关键影响。研究利用重组JUNO蛋白进行功能验证，证实特定糖链结构在受精中的调控作用，为生殖医学研究提供新靶点。
以上研究均涉及重组IZUMO1R/JUNO蛋白的表达与功能分析，涵盖受体发现、结构解析及相互作用机制，为理解受精过程及不孕症治疗奠定基础。
The IZUMO1R protein, also known as JUNO, is a cell surface receptor critical for mammalian fertilization. Discovered in 2014 as the cognate receptor for sperm-specific IZUMO1. it mediates the initial recognition and binding between gametes. This interaction represents one of the first essential steps in species-specific sperm-egg fusion, with both proteins requiring precise structural compatibility to enable successful fertilization.
Structurally, IZUMO1R belongs to the folate receptor family, featuring a glycosylphosphatidylinositol (GPI)-anchored extracellular domain. Its N-terminal region contains conserved folate-binding residues, though its primary biological role involves binding IZUMO1's immunoglobulin-like domain rather than folate metabolism. The receptor undergoes dynamic redistribution on the oolemma during oocyte maturation, clustering at the site of sperm interaction.
Recombinant IZUMO1R is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Purification often employs affinity tags combined with chromatographic techniques, yielding soluble extracellular domains that retain binding functionality. These recombinant proteins have become vital tools for studying gamete interaction mechanisms, enabling in vitro binding assays, structural studies via X-ray crystallography, and antibody development.
Research applications extend to fertility diagnostics and contraceptive development. Autoantibodies against IZUMO1R have been implicated in some cases of unexplained infertility, while engineered soluble receptors show potential as non-hormonal contraceptives by blocking sperm-egg binding. Current investigations focus on mapping critical binding epitopes and developing small-molecule inhibitors. The receptor's essential role in fertilization and species specificity makes it a compelling target for both reproductive medicine and evolutionary biology studies.
×
