| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPM |
| Uniprot No | P52272 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-730aa |
| 氨基酸序列 | AAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKE KNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYV ELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGE HARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGR LGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTF EQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGL GGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEG PFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGI ERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMG SGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPI DRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRM ATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALG AGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARK ACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFES PEVAERACRMMNGMKLSGREIDVRIDRNA |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNRNPM重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*HNRNPM regulates RNA processing and splicing during early mouse development*
**作者**：Li Y, et al.
**摘要**：该研究通过重组HNRNPM蛋白的体外实验，揭示了其在早期胚胎发育中调控RNA剪接和加工的关键作用，证明其通过结合特定RNA序列影响转录本稳定性。
2. **文献名称**：*Structural insights into the RNA recognition by the HNRNPM protein*
**作者**：Wang X, Zhang J.
**摘要**：利用重组HNRNPM蛋白的晶体结构解析，阐明了其RNA结合结构域(RRM)与靶RNA的相互作用机制，为理解其在RNA代谢中的分子基础提供依据。
3. **文献名称**：*HNRNPM promotes cancer metastasis through alternative splicing of integrin β1*
**作者**：Chen H, et al.
**摘要**：通过重组蛋白功能恢复实验，证明HNRNPM通过调控整合素β1的可变剪接促进肿瘤细胞侵袭转移，提示其作为癌症治疗靶点的潜力。
(注：以上文献信息为示例性质，实际引用需根据具体论文调整。)
HNRNPM (heterogeneous nuclear ribonucleoprotein M) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation. Structurally, it contains RNA recognition motifs (RRMs) and a glycine-rich domain, enabling interactions with RNA, DNA, and proteins. HNRNPM regulates alternative splicing by binding to exonic or intronic splicing enhancers/silencers, influencing transcript diversity. It also participates in post-transcriptional regulation, such as mRNA nuclear export and miRNA-mediated gene silencing.
Recombinant HNRNPM proteins are engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to study its molecular functions. These proteins retain functional domains and are often tagged (e.g., His, FLAG) for purification and detection. Researchers utilize recombinant HNRNPM to investigate RNA-protein interactions, splicing mechanisms, and its role in diseases. For example, HNRNPM dysregulation is linked to cancers (e.g., breast, lung) via aberrant splicing of oncogenes/tumor suppressors, and neurodegenerative disorders like Alzheimer’s, where it affects tau isoform balance.
In drug discovery, recombinant HNRNPM aids in screening compounds targeting RNA-protein interactions. Its study also sheds light on viral infections, as HNRNPM interacts with viral RNAs to modulate replication. Overall, recombinant HNRNPM serves as a vital tool for dissecting RNA biology and disease pathways, bridging structural insights with functional and therapeutic exploration.
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