| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCND1 |
| Uniprot No | Q9NSA2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 410-647aa |
| 氨基酸序列 | NFSRIYHQNQRADKRRAQQKVRLARIRLAKSGTTNAFLQYKQNGGLEDSGSGEEQALCVRNRSAFEQQHHHLLHCLEKTTCHEFTDELTFSEALGAVSPGGRTSRSTSVSSQPVGPGSLLSSCCPRRAKRRAIRLANSTASVSRGSMQELDMLAGLRRSHAPQSRSSLNAKPHDSLDLNCDSRDFVAAIISIPTPPANTPDESQPSSPGGGGRAGSTLRNSSLGTPCLFPETVKISSL |
| 预测分子量 | 29.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCND1重组蛋白的3篇参考文献示例(注：部分为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **标题**：Functional expression of the KCND1-encoded Kv4.1 potassium channel in mammalian cell lines
**作者**：Wang Y, et al.
**摘要**：该研究在HEK-293细胞中成功表达了重组KCND1(Kv4.1)通道蛋白，并利用膜片钳技术分析其电生理特性。结果显示，重组Kv4.1介导快速激活和失活的A型钾电流，其动力学特性受辅助亚基KChIP2调控，为研究心脏瞬时外向钾电流(Ito)提供了模型。
2. **标题**：Purification and biophysical characterization of recombinant KCND1 for structural studies
**作者**：Lee S, et al.
**摘要**：通过杆状病毒-昆虫细胞系统表达并纯化KCND1重组蛋白，结合冷冻电镜技术解析其三维结构。研究发现，Kv4.1的电压敏感结构域在失活过程中发生构象变化，揭示了其快速失活的分子机制。
3. **标题**：KCND1 mutations alter channel trafficking studied via recombinant expression in neurons
**作者**：Chen X, et al.
**摘要**：利用神经元模型表达KCND1重组蛋白，探讨致病性突变(如R67W)对通道膜转运的影响。结果表明，突变导致蛋白滞留于内质网，降低细胞膜电流密度，提示其在神经系统疾病中的潜在机制。
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**说明**：以上文献为示例性质，实际研究中建议通过PubMed、Google Scholar等平台以关键词“KCND1 recombinant”“Kv4.1 expression”检索最新文献，并优先选择近年的结构生物学或疾病机制研究。
KCND1 recombinant protein is derived from the KCND1 gene, which encodes the voltage-gated potassium channel subunit Kv4.1. This protein belongs to the Shal-type subfamily of potassium channels and plays a critical role in regulating neuronal and cardiac excitability. Kv4.1 channels contribute to the transient outward potassium current (Ito), which is essential for repolarizing membrane potentials during action potentials, particularly in the heart and brain. In cardiomyocytes, Kv4.1-mediated currents help shape early repolarization phases, influencing heart rhythm and electrical stability. Dysregulation of Kv4.1 has been linked to arrhythmias and neurological disorders.
Recombinant KCND1 protein is produced using heterologous expression systems (e.g., HEK293 cells or *Xenopus* oocytes) to study channel properties, drug interactions, and disease mechanisms. Its structure includes six transmembrane domains, a voltage-sensing domain, and a pore-forming region. Researchers often co-express it with auxiliary subunits like KChIPs (Kv Channel-Interacting Proteins) to replicate native channel behavior. This protein is crucial for electrophysiological studies, such as patch-clamp experiments, to explore kinetic properties, pharmacology, and mutations (e.g., those linked to Brugada syndrome).
Additionally, KCND1 recombinant proteins serve as antigens for antibody development or tools for high-throughput screening of ion channel modulators. Understanding Kv4.1's role in health and disease aids in developing targeted therapies for cardiac arrhythmias, epilepsy, or neurodegenerative conditions. Its study also sheds light on ion channel evolution and functional diversity within the nervous and cardiovascular systems.
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