| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT10 |
| Uniprot No | P13645 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY |
| 预测分子量 | 62.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT10重组蛋白的模拟参考文献示例(请注意，以下内容为虚构，仅用于演示格式)：
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1. **标题**：*Recombinant Human Keratin 10: Expression in E. coli and Characterization*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统高效表达重组人KRT10蛋白，并优化纯化流程。结果显示重组蛋白具有天然KRT10的免疫反应性，可用于体外皮肤屏障功能研究。
2. **标题**：*Structural Analysis of KRT10 Mutants Using Recombinant Protein Technology*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞表达系统生成KRT10突变体重组蛋白，结合X射线晶体学解析其结构，揭示特定突变导致表皮松解性角化过度的分子机制。
3. **标题**：*KRT10 Recombinant Protein in Psoriasis-like Inflammation Models*
**作者**：Wang Y, et al.
**摘要**：在银屑病样细胞模型中评估重组KRT10的抗炎作用，发现其通过调节NF-κB通路减轻炎症反应，提示潜在治疗价值。
4. **标题**：*Interaction of KRT10 with Epidermal Lipids: Insights from Recombinant Protein Assays*
**作者**：Jones R, et al.
**摘要**：通过表面等离子体共振(SPR)技术分析重组KRT10与表皮脂质的相互作用，阐明其在皮肤屏障形成中的关键结合特性。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“KRT10 recombinant protein”获取最新研究。
Keratins are a family of structural proteins critical for maintaining the integrity of epithelial cells. KRT10. a type I keratin (acidic), pairs with KRT1 (type II, basic) to form intermediate filaments in the suprabasal layers of the epidermis. This heterodimer provides mechanical strength and resilience to skin, protecting against mechanical stress and dehydration. Mutations in the KRT10 gene are linked to skin disorders like epidermolytic hyperkeratosis (EHI) and ichthyosis with confetti (IWC), characterized by abnormal keratinocyte differentiation, skin fragility, and hyperkeratosis.
Recombinant KRT10 protein is produced using expression systems (e.g., bacteria, mammalian cells) to study its biological roles and disease mechanisms. Its production involves cloning the KRT10 gene into vectors, followed by purification via chromatography. This allows researchers to obtain pure, functional protein for structural analysis, interaction studies, and drug screening. Recombinant KRT10 is particularly valuable for investigating mutation-induced filament aggregation, a hallmark of keratinopathies, and testing therapeutic agents aiming to restore normal filament assembly.
In translational research, recombinant KRT10 aids in developing cell or tissue models to mimic epidermal defects. It also serves as an antigen for antibody production in diagnostic assays. Beyond dermatology, it contributes to understanding epithelial cancers, as keratin expression patterns often shift during tumor progression. Despite challenges in maintaining solubility during recombinant production (due to its insoluble native structure), advances in solubilization tags and refolding techniques have enhanced its utility. Overall, recombinant KRT10 remains a key tool in decoding keratin biology and advancing therapies for keratin-related disorders.
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