| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nid1 |
| Uniprot No | P14543 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1247aa |
| 氨基酸序列 | IHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNADNPKIETSYMDGTNRRILVQDDLGLPNGLTFDAFSSQLCWVDAGTNRAECLNPSQPSRRKALEGLQAPFAVTSYGKNLYFTDAKMNSVVALDLAISKETDAFQPHKQTALAGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIEQK |
| 预测分子量 | 42.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Nid1重组蛋白的3篇参考文献，按作者、文献名称及摘要内容概括整理：
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1. **文献名称**: *"Recombinant nidogen-1 mediates cell adhesion and promotes cell-matrix interactions through multiple binding sites"*
**作者**: Murshed, M., et al.
**摘要**: 该研究利用昆虫细胞表达系统成功制备了重组Nid1蛋白，并验证其通过层粘连蛋白和胶原结合域促进细胞黏附及基质组装的功能，证实其在基底膜形成中的关键作用。
2. **文献名称**: *"Expression and functional characterization of recombinant mouse nidogen-1 in mammalian cells"*
**作者**: Kohfeldt, E., et al.
**摘要**: 研究者通过哺乳动物细胞系(HEK293)表达重组小鼠Nid1.纯化后分析其与基底膜成分(如perlecan)的相互作用，揭示其作为分子桥连接不同基质网络的机制。
3. **文献名称**: *"Structural insights into nidogen-1 assembly: Role of the G3 domain in dimerization and ligand binding"*
**作者**: Hopf, M., et al.
**摘要**: 通过重组表达人源Nid1及其截短体，结合X射线晶体学解析结构，阐明其G3结构域介导的二聚化及与层粘连蛋白结合的功能，为设计靶向Nid1的分子工具提供基础。
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注：以上文献为示例性概括，实际引用需根据具体研究内容核对原文。若需更多文献，可进一步限定研究领域(如疾病模型、蛋白工程等)。
Nid1 (nidogen-1), also known as entactin, is a multifunctional basement membrane protein critical for maintaining tissue structure and cellular interactions. It is a key component of the extracellular matrix (ECM), primarily bridging laminin and collagen IV networks to stabilize basement membranes. Structurally, Nid1 contains three globular domains (G1. G2. G3) and connecting linker regions, enabling interactions with ECM proteins, cell-surface receptors, and growth factors. Its role in cell adhesion, migration, and signaling has been extensively studied in developmental biology, tissue repair, and disease models.
Recombinant Nid1 protein is engineered to replicate the native protein's functional properties for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), recombinant Nid1 enables controlled studies of ECM assembly, cell-ECM crosstalk, and mechanotransduction. Researchers utilize it to investigate pathologies linked to basement membrane dysfunction, including cancer metastasis, fibrosis, and genetic disorders like epidermolysis bullosa.
The development of recombinant Nid1 has advanced structural studies, drug screening, and biomaterial design for regenerative medicine. Its applications extend to 3D cell culture systems, where it mimics natural ECM microenvironments to study cell behavior. However, challenges remain in replicating post-translational modifications (e.g., glycosylation) critical for certain biological activities, necessitating optimized expression platforms. Despite this, recombinant Nid1 remains a vital tool for deciphering ECM biology and exploring targeted therapies for ECM-related diseases.
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