| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RANBP2 |
| Uniprot No | P49792 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2601-2802aa |
| 氨基酸序列 | PTEESSINYTFKTPEKAKEKKKPEDSPSDDDVLIVYELTPTAEQKALATKLKLPPTFFCYKNRPDYVSEEEEDDEDFETAVKKLNGKLYLDGSEKCRPLEENTADNEKECIIVWEKKPTVEEKAKADTLKLPPTFFCGVCSDTDEDNGNGEDFQSELQKVQEAQKSQTEEITSTTDSVYTGGTEVMVPSFCKSEEPDSITKS |
| 预测分子量 | 24.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RANBP2重组蛋白的参考文献示例(均为虚构，仅供格式参考)：
1. **标题**: "RANBP2 facilitates viral replication by interacting with HIV-1 Rev protein"
**作者**: Smith A, et al.
**摘要**: 研究利用重组RANBP2蛋白揭示其通过核质转运机制促进HIV-1 Rev蛋白介导的病毒RNA输出，为抗病毒靶点提供新思路。
2. **标题**: "Structural analysis of RANBP2 zinc finger domain in nucleocytoplasmic transport"
**作者**: Lee JH, et al.
**摘要**: 通过重组RANBP2蛋白的晶体结构解析，阐明其锌指结构域在结合RanGTP和核转运受体中的作用机制。
3. **标题**: "RANBP2 mutations alter SUMOylation activity in familial acute necrotizing encephalopathy"
**作者**: García M, et al.
**摘要**: 发现RANBP2基因突变导致重组蛋白SUMO连接酶活性异常，与急性坏死性脑病(ANE)的发病机制密切相关。
4. **标题**: "RANBP2 recombinant protein rescues mitotic defects in RANBP2-depleted cells"
**作者**: Chen X, et al.
**摘要**: 体外实验表明，重组RANBP2蛋白可恢复有丝分裂中纺锤体组装异常，证实其在维持基因组稳定性中的关键功能。
*注：以上文献为示例，实际研究请通过PubMed或Google Scholar检索关键词(如“RANBP2 recombinant protein”)获取最新数据。*
**Background of RANBP2 Recombinant Protein**
RANBP2 (RAN Binding Protein 2), also known as Nup358. is a critical component of the nuclear pore complex (NPC), which mediates nucleocytoplasmic transport. It is a large, multidomain protein located on the cytoplasmic filaments of the NPC and plays essential roles in coordinating molecular trafficking, mitosis, and SUMOylation processes. Structurally, RANBP2 contains distinct domains, including zinc finger motifs, leucine-rich repeats, a cyclophilin-homologous region, and a SUMO-binding domain, enabling interactions with transport factors (e.g., importin-β, exportin-1), SUMO-modified proteins, and molecular chaperones.
As an E3 SUMO ligase, RANBP2 facilitates the SUMOylation of target proteins like RanGAP1. a regulator of RAN GTPase activity critical for maintaining nucleocytoplasmic transport gradients. Dysregulation of RANBP2 is linked to diseases, including acute necrotizing encephalopathy (ANE), where mutations impair its SUMOylation function, leading to neurological pathologies.
Recombinant RANBP2 proteins are engineered using heterologous expression systems (e.g., bacteria, mammalian cells) to study its biochemical properties, interactions, and structural dynamics. These tools enable in vitro analyses of SUMOylation mechanisms, NPC assembly, and RAN GTPase regulation. Additionally, recombinant RANBP2 aids in exploring disease mechanisms and screening therapeutic compounds targeting SUMOylation pathways. Its modular domains are also utilized in synthetic biology for designing hybrid proteins or engineering cellular transport systems.
Despite its complexity, recombinant RANBP2 remains pivotal for dissecting NPC functions, intracellular signaling, and developing interventions for NPC-associated disorders.
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