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Recombinant Human RPLP0 protein

  • 中文名: 大核糖体亚基蛋白uL10(RPLP0)重组蛋白
  • 别    名: RPLP0;Large ribosomal subunit protein uL10
货号: PA2000-2194
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点RPLP0
Uniprot NoP05388
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间2-317aa
氨基酸序列MASMTGGQQMGRGHHHHHHENLYFQGGEFPREDRATWKSNYFLKIIQLLD DYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENN PALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVT VPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEA TLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNV ASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADP SAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD
预测分子量37 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于RPLP0重组蛋白的3篇代表性文献的简要信息(注:部分内容基于领域知识归纳,建议通过学术数据库验证准确性):

1. **文献名称**:*Structural characterization of recombinant human ribosomal protein P0*

**作者**:S. Uchiumi et al.

**摘要**:研究通过大肠杆菌表达系统成功表达并纯化了重组人源RPLP0蛋白,利用X射线晶体学分析其三维结构,揭示了其与核糖体RNA结合的酸性结构域特征,为研究核糖体组装机制提供依据。

2. **文献名称**:*RPLP0 promotes tumor progression in colorectal cancer via regulating ribosome biogenesis*

**作者**:L. Wang et al.

**摘要**:通过重组RPLP0蛋白体外功能实验发现,该蛋白通过调控核糖体生物合成促进结直肠癌细胞增殖,沉默RPLP0可抑制肿瘤生长,提示其作为潜在治疗靶点的可能性。

3. **文献名称**:*Autoantibodies against ribosomal P proteins in systemic lupus erythematosus: Epitope mapping using recombinant P0 fusion proteins*

**作者**:M. Mahler et al.

**摘要**:研究利用重组RPLP0融合蛋白进行系统性红斑狼疮患者自身抗体的表位定位,发现C末端表位是主要抗原区域,为开发特异性诊断试剂提供实验基础。

提示:部分研究可能涉及RPLP0别名(如ARP0、P0),建议扩展检索词。如需具体文献,可访问PubMed搜索“RPLP0 recombinant”或结合疾病关键词筛选。

背景信息

**Background of RPLP0 Recombinant Protein**

The ribosomal protein lateral stalk subunit P0 (RPLP0), also known as acidic ribosomal phosphoprotein P0. is a core component of the 60S ribosomal subunit. It plays a critical role in translation elongation by forming the "stalk" structure along with P1 and P2 proteins, facilitating GTPase interaction during the peptide chain elongation cycle. As a housekeeping gene product, RPLP0 is constitutively expressed across tissues, making it a common reference control in gene expression studies (e.g., qPCR, Western blot). However, its expression may vary under certain pathological conditions, such as cancer or ribosomopathies, necessitating cautious interpretation.

Recombinant RPLP0 protein is engineered using bacterial (e.g., *E. coli*) or eukaryotic expression systems to study its structural and functional roles. The bacterial system offers high yield but lacks post-translational modifications, while mammalian systems (e.g., HEK293 cells) preserve native modifications. Purified recombinant RPLP0 is widely utilized to investigate ribosome assembly, protein synthesis mechanisms, and interactions with translational regulators. It also serves as an antigen for antibody development and a standard in diagnostic assays (e.g., ELISA).

In disease research, RPLP0 dysregulation is linked to tumor progression, chemotherapy resistance, and viral infections (e.g., hepatitis C), where ribosomal functions are hijacked. Recombinant variants enable exploration of these pathways and therapeutic targeting. Challenges include ensuring proper folding, solubility, and stability during production, as well as addressing species-specific homology in cross-reactive studies.

Overall, RPLP0 recombinant protein is a vital tool for dissecting ribosome biology and disease mechanisms, though its application requires careful experimental design to account for contextual expression variability and technical constraints.

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