| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX20 |
| Uniprot No | Q7Z614 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-102aa |
| 氨基酸序列 | MASPEHPGSPGCMGPITQCTARTQQEAPATGPDLPHPGPDGHLDTHSGLSSNSSMTTRELQQYWQNQKCRWKHVKLLFEIASARIEERKVSKFVVYQIIVIQ |
| 预测分子量 | 38.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX20重组蛋白的3篇参考文献概览(文献信息为虚构示例，仅供参考格式)：
1. **文献名称**：*SNX20 regulates TLR4 signaling by mediating endosomal trafficking of the receptor complex*
**作者**：Jia Y, et al.
**摘要**：该研究通过表达SNX20重组蛋白，揭示其在调控TLR4信号通路中的作用，证明SNX20通过介导TLR4内体分选和运输影响NF-κB激活，从而调节先天免疫反应。
2. **文献名称**：*Structural characterization of SNX20 BAR domain and its role in membrane curvature sensing*
**作者**：Zhang L, et al.
**摘要**：利用重组SNX20蛋白进行结构解析，发现其BAR结构域通过识别磷脂膜曲率参与囊泡形成，为SNX家族蛋白的膜重塑机制提供了分子基础。
3. **文献名称**：*SNX20 interacts with VPS34 to modulate autophagosome maturation*
**作者**：Xu H, et al.
**摘要**：通过体外重组蛋白结合实验，证明SNX20与VPS34复合物互作，调控自噬体成熟过程，影响细胞在营养胁迫下的自噬通量。
注：以上文献及内容为模拟示例，实际研究需查阅真实数据库(如PubMed)。若需真实文献，建议通过学术引擎检索关键词“SNX20 recombinant protein”或“SNX20 function”。
**Background of SNX20 Recombinant Protein**
SNX20 (Sorting Nexin 20) is a member of the sorting nexin family, a group of proteins characterized by their phosphoinositide-binding PX domains. These proteins play critical roles in membrane trafficking, endosomal sorting, and cellular signaling. SNX20 is distinctively involved in regulating immune responses, particularly through its interaction with Toll-like receptor (TLR) signaling pathways. It localizes to early endosomes, where it facilitates the trafficking and activation of TLRs, thereby modulating innate immune reactions to pathogens. Studies suggest SNX20 also influences macrophage function and inflammatory cytokine production, linking it to autoimmune diseases and chronic inflammation.
Recombinant SNX20 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. The recombinant form retains functional domains, including the PX domain, enabling researchers to study its lipid-binding properties, protein interactions, and regulatory mechanisms *in vitro*.
Research applications include elucidating SNX20’s role in TLR signaling dysregulation, exploring its potential as a therapeutic target for inflammatory disorders, and deciphering its impact on endosomal-lysosomal pathways in cancer metastasis. Its recombinant variant is also used to generate antibodies for diagnostic assays or to screen small-molecule inhibitors. Despite progress, challenges remain in understanding its tissue-specific functions and crosstalk with other sorting nexins. Overall, SNX20 recombinant protein serves as a vital tool for dissecting immune regulation and membrane dynamics in health and disease.
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