| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNPH |
| Uniprot No | O15079 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MAMSLPGSRRTSAGSRRRTSPPVSVRDAYGTSSLSSSSNSGSYKGSDSSPTPRRSMKYTLCSDNHGIKPPTPEQYLTPLQQKEVCIRHLKARLKDTQDRLQDRDTEIDDLKTQLSRMQEDWIEEECHRVEAQLALKEARKEIKQLKQVIDTVKNNLIDKDKGLQKYFVDINIQNKKLETLLHSMEVAQNGMAKEDGTGESAGGSPARSLTRSSTYTKLSDPAVCGDRQPGDPSSGSAEDGADSGFAAADDTLSRTDALEASSLLSSGVDCGTEETSLHSSFGLGPRFPASNTYEKLLCGMEAGVQASCMQERAIQTDFVQYQPDLDTILEKVTQAQVCGTDPESGDRCPELDAHPSGPRDPNSAVVVTVGDELEAPEPITRGPTPQRPGANPNPGQSVSVVCPMEEEEEAAVAEKEPKSYWSRH |
| 预测分子量 | 62.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SNPH(Syntaphilin)重组蛋白相关的文献概览：
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1. **文献名称**：*Syntaphilin regulates mitochondrial dynamics and neurodegeneration*
**作者**：Kang, J.S., et al.
**摘要**：研究揭示了SNPH通过控制线粒体锚定与运输维持神经元能量稳态的机制，利用重组SNPH蛋白验证其在抑制线粒体过度移动中的作用，为神经退行性疾病提供新靶点。
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2. **文献名称**：*Recombinant Syntaphilin enhances axonal mitochondrial localization and mitigates Parkinson’s pathology*
**作者**：Chen, Y., et al.
**摘要**：开发了高效表达重组SNPH蛋白的方法，证明其可促进轴突内线粒体锚定，改善帕金森病模型中神经元功能，为基因治疗提供实验依据。
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3. **文献名称**：*Structural insights into Syntaphilin-mediated mitochondrial docking*
**作者**：Smith, L.M., et al.
**摘要**：通过重组SNPH蛋白的晶体结构解析，阐明其与微管及线粒体膜蛋白的相互作用位点，揭示SNPH调控线粒体空间分布的分子机制。
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(注：以上文献为虚拟示例，实际文献需通过学术数据库检索确认。)
**Background of SNPH (Syntaphilin) Recombinant Protein**
Syntaphilin (SNPH) is a mitochondrial-associated protein that plays a critical role in regulating mitochondrial trafficking and anchoring in neurons. Discovered in the early 2000s, SNPH acts as a "static anchor" by tethering mitochondria to the cytoskeleton, particularly in energy-demanding regions such as synaptic terminals. This function is vital for maintaining mitochondrial homeostasis, ensuring localized energy supply, calcium buffering, and synaptic plasticity in neuronal cells. Dysregulation of SNPH has been linked to neurodegenerative disorders, including Alzheimer’s disease and Parkinson’s disease, where impaired mitochondrial dynamics contribute to neuronal dysfunction.
Recombinant SNPH protein is engineered using molecular cloning techniques, where the SNPH gene is expressed in heterologous systems like *E. coli* or mammalian cell cultures. This allows large-scale production of purified, functional SNPH for research applications. Recombinant SNPH retains its ability to interact with microtubules and motor proteins (e.g., kinesin), enabling studies on mitochondrial transport mechanisms. Researchers utilize it to investigate SNPH’s role in neuronal health, mitochondrial positioning under stress, and its interplay with apoptotic signaling pathways.
Furthermore, SNPH recombinant protein has therapeutic potential. By modulating mitochondrial anchoring, it may help address pathologies caused by mitochondrial mislocalization. Current studies explore its utility in neuroprotection, cancer (where mitochondrial dynamics influence metastasis), and metabolic diseases. Despite progress, challenges remain in understanding SNPH’s post-translational modifications and tissue-specific regulation. Ongoing research aims to refine recombinant SNPH tools for high-resolution structural studies and drug screening, advancing both basic science and translational medicine.
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