| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Sprr2a1 |
| Uniprot No | Q9CQK8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-83aa |
| 氨基酸序列 | MSYYQQQCNQPCRPPPVCPPPKCPEPCPPQVWPGPCRPVMCFEPCLPSVWPGPCRPVVCYEQCPPQPWQSTCPPVQFPPCQQK |
| 预测分子量 | 13.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Sprr2a1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"SPRR2A1 recombinant protein enhances keratinocyte differentiation and skin barrier repair"*
**作者**: Smith J, et al.
**摘要**: 研究通过体外表达Sprr2a1重组蛋白，发现其促进人角质形成细胞分化和屏障相关蛋白(如loricrin)的表达，提示其在皮肤屏障修复中的潜在治疗作用。
2. **文献名称**: *"Pro-inflammatory role of recombinant SPRR2A1 in airway epithelial cells"*
**作者**: Lee H, et al.
**摘要**: 实验表明，Sprr2a1重组蛋白通过激活NF-κB通路增强气道上皮细胞IL-8分泌，可能参与慢性呼吸道炎症的病理机制。
3. **文献名称**: *"Recombinant SPRR2A1 binds to lipid membranes and protects against oxidative stress"*
**作者**: Müller R, et al.
**摘要**: 通过体外重构实验，发现Sprr2a1重组蛋白通过与细胞膜磷脂结合，减少活性氧(ROS)损伤，提示其抗氧化保护功能。
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注：以上文献为示例性质，实际引用需以具体发表文章为准。建议通过PubMed或Web of Science以关键词“Sprr2a1 recombinant”检索最新研究。
**Background of SPRR2A1 Recombinant Protein**
The Small Proline-Rich Protein 2A1 (SPRR2A1) is a member of the SPRR protein family, which is primarily associated with epidermal differentiation and barrier formation. These cysteine-rich, calcium-binding proteins play critical roles in strengthening the cornified envelope—a specialized structure in the outermost skin layer that protects against environmental stressors, pathogens, and water loss. SPRR2A1 is specifically expressed in keratinocytes during late differentiation, where it contributes to crosslinking proteins and lipids to ensure mechanical resilience and impermeability. Its expression is upregulated in response to skin injury, inflammation, or exposure to UV radiation, highlighting its role in stress adaptation and tissue repair.
Recombinant SPRR2A1 protein is engineered using genetic cloning techniques, often expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. The purified protein retains functional domains necessary for studying its interactions with other cornified envelope components, such as loricrin and involucrin. Researchers utilize SPRR2A1 recombinant protein to investigate epidermal homeostasis, barrier dysfunction in diseases like psoriasis or atopic dermatitis, and mechanisms of wound healing.
Beyond dermatology, SPRR2A1 has been explored in cancer research due to its aberrant expression in certain carcinomas, suggesting potential as a diagnostic biomarker or therapeutic target. Its role in oxidative stress response and extracellular matrix remodeling also extends its relevance to inflammatory and fibrotic disorders. By enabling in vitro and in vivo functional studies, recombinant SPRR2A1 serves as a vital tool for unraveling skin biology and developing targeted therapies for epithelial pathologies.
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