| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Trove2 |
| Uniprot No | P10155 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-376aa |
| 氨基酸序列 | IANSQ DGYVWQVTDM NRLHRFLCFG SEGGTYYIKE QKLGLENAEA LIRLIEDGRG CEVIQEIKSF SQEGRTTKQE PMLFALAICS QCSDISTKQA AFKAVSEVCR IPTHLFTFIQ FKKDLKESMK CGMWGRALRK AIADWYNEKG GMALALAVTK YKQRNGWSHK DLLRLSHLKP SSEGLAIVTK YITKGWKEVH ELYKEKALSV ETEKLLKYLE AVEKVKRTRD ELEVIHLIEE HRLVREHLLT NHLKSKEVWK ALLQEMPLTA LLRNLGKMTA NSVLEPGNSE VSLVCEKLCN EKLLKKARIH PFHILIALET YKTGHGLRGK LKWRPDEEIL KALDAAFYKT FKTVEPTGKR FLLAVD |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TROVE2(Ro60)重组蛋白的示例参考文献(文献信息为示例性虚构，仅供格式参考)：
1. **文献名称**：Structural and Functional Analysis of Recombinant TROVE2 Protein in Autoimmune Response
**作者**：Smith J. et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化重组TROVE2蛋白，通过X射线晶体学解析其三维结构，证实其与Y RNA结合的能力，并探讨其在系统性红斑狼疮(SLE)患者血清中的自身抗体识别机制。
2. **文献名称**：Expression and Immunogenicity of Recombinant TROVE2 in Lupus Mouse Models
**作者**：Chen L. et al.
**摘要**：利用哺乳动物细胞系统表达重组TROVE2蛋白，证明其在诱导小鼠模型产生抗Ro60抗体的过程中起关键作用，为研究SLE发病机制提供了实验依据。
3. **文献名称**：TROVE2 Recombinant Protein as a Diagnostic Marker for Sjögren's Syndrome
**作者**：Wang Y. et al.
**摘要**：通过真核表达系统制备高纯度TROVE2重组蛋白，用于ELISA检测，发现其在干燥综合征患者血清中具有高特异性抗体反应，提示其临床诊断潜力。
4. **文献名称**：Mechanistic Insights into TROVE2-RNA Interactions Using Recombinant Protein Mutants
**作者**：Kim S. et al.
**摘要**：构建TROVE2重组蛋白的突变体，通过体外结合实验揭示其RNA结合结构域的关键氨基酸残基，阐明其在细胞应激颗粒形成中的调控作用。
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注：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以关键词“TROVE2 recombinant protein”、“Ro60 expression”等检索真实文献。
**Background of Trove2 Recombinant Protein**
Trove2 (TROVE domain family member 2), also known as Ro60 or SSA2. is a multifunctional RNA-binding protein encoded by the *TROVE2* gene. It is a member of the TROVE domain protein family, characterized by a conserved ~60-amino acid TROVE motif involved in nucleic acid interactions. Structurally, Trove2 forms a ring-shaped complex and associates with non-coding RNAs, such as Y RNAs, to facilitate ribonucleoprotein (RNP) assembly. This protein plays a role in RNA quality control, including the recognition and degradation of misfolded or damaged RNAs, and is implicated in cellular stress responses, autoimmune regulation, and viral defense mechanisms.
Trove2 is best known as an autoantigen in systemic autoimmune diseases, particularly systemic lupus erythematosus (SLE), where antibodies against Trove2 are commonly detected. Its recombinant form is engineered for research and therapeutic applications. Recombinant Trove2 proteins are typically produced in *E. coli* or mammalian expression systems, ensuring high purity and consistency for experimental use. These proteins retain key functional domains, enabling studies on RNA-protein interactions, autoimmune pathogenesis, and molecular pathways linked to Trove2’s biological roles.
In research, recombinant Trove2 serves as a tool to investigate its structure-function relationships, epitope mapping for autoantibody detection, and its interplay with RNAs or immune system components. It also holds potential in developing diagnostic assays and targeted therapies for autoimmune disorders. By leveraging recombinant technology, Trove2 studies continue to advance our understanding of its dual roles in cellular homeostasis and disease, bridging gaps between RNA biology and immune dysregulation.
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