| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF155 |
| Uniprot No | Q12901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538aa |
| 氨基酸序列 | MTTFKEAVTFKDVAVVFTEEELGLLDPAQRKLYRDVMLENFRNLLSVGHQPFHQDTCHFLREEKFWMMGTATQREGNSGGKIQTELESVPEAGAHEEWSCQQIWEQIAKDLTRSQDSIINNSQFFENGDVPSQVEAGLPTIHTGQKPSQGGKCKQSISDVPIFDLPQQLYSEEKSYTCDECGKSICYISALHVHQRVHVGEKLFMCDVCGKEFSQSSHLQTHQRVHTGEKPFKCEQCGKGFSRRSALNVHRKLHTGEKPYICEACGKAFIHDSQLKEHKRIHTGEKPFKCDICGKTFYFRSRLKSHSMVHTGEKPFRCDTCDKSFHQRSALNRHCMVHTGEKPYRCEQCGKGFIGRLDFYKHQVVHTGEKPYNCKECGKSFRWSSCLLNHQRVHSGEKSFKCEECGKGFYTNSQLSSHQRSHSGEKPYKCEECGKGYVTKFNLDLHQRVHTGERPYNCKECGKNFSRASSILNHKRLHCQKKPFKCEDCGKRLVHRTYRKDQPRDYSGENPSKCEDCGRRYKRRLNLDILLSLFLNDT |
| 预测分子量 | 78.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于ZNF155重组蛋白的研究文献较少，以下列举部分相关文献信息供参考(注：文献标题和内容为模拟概括，实际研究需以真实数据库检索为准)：
1. **"Cloning and characterization of ZNF155: A novel human zinc finger protein"**
*Author: Zhang Y, et al.*
*摘要*：首次报道了ZNF155基因的克隆和重组蛋白表达，发现其属于KRAB-ZFP家族，可能在转录调控中发挥作用。
2. **"Expression and purification of recombinant ZNF155 in E. coli for structural studies"**
*Author: Lee S, et al.*
*摘要*：开发了ZNF155重组蛋白在大肠杆菌中的高效表达和纯化方法，并分析了其锌指结构域的稳定性。
3. **"ZNF155 interacts with histone deacetylases and regulates gene silencing"**
*Author: Müller P, et al.*
*摘要*：通过重组蛋白互作实验，发现ZNF155与HDAC复合物结合，可能参与表观遗传调控和肿瘤抑制通路。
4. **"Functional analysis of ZNF155 mutations in neurodevelopmental disorders"**
*Author: Chen H, et al.*
*摘要*：利用重组ZNF155蛋白进行体外功能实验，揭示了某些突变体导致DNA结合能力丧失，可能与神经系统疾病相关。
**提示**：ZNF155研究相对较少，建议通过PubMed或Google Scholar结合关键词(如"ZNF155 recombinant"、"ZNF155 protein function")检索最新文献。部分研究可能集中于锌指蛋白家族共性机制，而非单独聚焦ZNF155.
ZNF155 is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, which represents one of the largest groups of transcription regulators in humans. These proteins are characterized by tandem arrays of C2H2 zinc finger motifs responsible for DNA binding and a KRAB domain at the N-terminus that often mediates transcriptional repression through interactions with chromatin-modifying complexes. ZNF155. encoded by the *ZNF155* gene on human chromosome 19. is thought to play roles in gene regulation, though its specific biological functions remain less characterized compared to related family members like ZNF267 or ZNF274.
Recombinant ZNF155 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Typically produced in bacterial (e.g., *E. coli*) or eukaryotic (e.g., mammalian or insect cell) expression systems, the recombinant protein retains functional domains for DNA binding and protein-protein interactions. Purification methods, such as affinity chromatography with His-tags or GST tags, ensure high yield and purity for experimental use.
Research on recombinant ZNF155 focuses on its potential involvement in epigenetic regulation, genome stability, or developmental processes, leveraging techniques like electrophoretic mobility shift assays (EMSA) to map DNA-binding motifs or co-immunoprecipitation to identify interacting partners. Its aberrant expression has been tentatively linked to diseases such as cancers or neurological disorders, though mechanistic insights are still emerging. The protein’s modular structure also makes it a candidate for synthetic biology applications, including engineered zinc finger nucleases (ZFNs) for targeted genome editing. Overall, ZNF155 recombinant protein serves as a critical tool to decode the functional diversity of KRAB-ZFPs and their impact on human health.
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