| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF160 |
| Uniprot No | Q9HCG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MALTQVRLTFRDVAIEFSQEEWKCLDPAQRILYRDVMLENYWNLVSLGLCHFDMNIISMLEEGKEPWTVKSCVKIARKPRTPECVKGVVTDLLRRWKHWLLLLGICCPKPHGRVSSRLRLSRSLGHFFHSAFATFMGVCDKRVGSIF |
| 预测分子量 | 22.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF160重组蛋白的虚构参考文献示例(仅供模拟参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *Cloning and Prokaryotic Expression of Human ZNF160 Recombinant Protein*
**作者**: Zhang L., et al.
**摘要**: 本研究成功克隆了人源ZNF160基因编码区，构建了原核表达载体，并在大肠杆菌中诱导表达。通过优化IPTG浓度和温度，获得可溶性重组ZNF160蛋白，为后续功能研究提供基础。
2. **文献名称**: *Functional Analysis of ZNF160 Recombinant Protein in Transcriptional Regulation*
**作者**: Kim S., Park J.H.
**摘要**: 利用哺乳动物细胞系统表达ZNF160重组蛋白，通过荧光素酶报告基因实验证实其结合特定DNA序列并抑制靶基因转录，提示其在细胞分化中可能发挥调控作用。
3. **文献名称**: *Structural Characterization of ZNF160 Zinc Finger Domains by Recombinant Protein Expression*
**作者**: Müller F., et al.
**摘要**: 采用昆虫杆状病毒系统表达高纯度ZNF160重组蛋白，通过圆二色光谱和X射线晶体学解析其锌指结构域的三维构象，揭示其与DNA结合的分子机制。
4. **文献名称**: *ZNF160 Recombinant Protein Attenuates Tumor Cell Proliferation in vitro*
**作者**: Chen X., et al.
**摘要**: 研究发现外源性添加ZNF160重组蛋白可抑制肝癌细胞HepG2的增殖，并通过RNA-seq分析表明其可能通过调控Wnt/β-catenin通路发挥作用。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台检索(可使用关键词“ZNF160 recombinant”或“ZNF160 protein expression”)。如需真实文献辅助，请提供更多研究背景或具体需求。
**Background of ZNF160 Recombinant Protein**
Zinc finger protein 160 (ZNF160) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2)-type zinc finger domains that facilitate DNA or RNA binding. These proteins are critical regulators of gene expression, often acting as transcription factors to modulate cellular processes such as differentiation, proliferation, and apoptosis. ZNF160. specifically, is encoded by the *ZNF160* gene located on human chromosome 19. and its expression has been implicated in various biological and pathological contexts, including cancer.
The recombinant ZNF160 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for research applications. Recombinant technology enables precise control over protein structure and post-translational modifications, enhancing its utility in mechanistic studies. Structurally, ZNF160 contains multiple tandem zinc finger motifs, which likely mediate sequence-specific interactions with nucleic acids. Some studies suggest its involvement in transcriptional repression or activation, potentially through recruitment of chromatin-modifying complexes.
Functionally, ZNF160 has been linked to tumor suppression in certain cancers. For example, reduced ZNF160 expression correlates with poor prognosis in hepatocellular carcinoma and gastric cancer, possibly due to its role in inhibiting oncogenic pathways or promoting apoptosis. Additionally, epigenetic silencing of *ZNF160* via hypermethylation has been observed in malignancies, highlighting its regulatory importance.
Researchers utilize recombinant ZNF160 protein to investigate its DNA-binding specificity, protein-protein interactions, and downstream target genes. Such studies aim to elucidate its molecular mechanisms in development and disease, offering potential therapeutic insights. Despite progress, the full scope of ZNF160's biological roles remains under exploration, necessitating further structural and functional analyses.
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