| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | all1616 |
| Uniprot No | Q8YWJ |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MRWVDDSYLLPAAKGLMSTSVPNTYAKLAKALLINYSFDLSGYHVDELVNRWQKQYPADWLHLAVIEALYQGRYKAISVQQLLAFWQRRGQEIYHFNMEFERLICSKFPESLTPMAASEQYSRQGKNQNQTLQLMSFKQQEQVKEEEEPPTEKMLALSSTSVTASIEVSVSQQEDYLGQPFSLNPDISTKLLPISVTHPPIGQFTPQTSDRSESFTSKLKAISNENS |
| 预测分子量 | 25,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **All1616 重组蛋白** 的3篇参考文献的简要信息，基于公开研究数据整理：
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1. **标题**: *Functional Characterization of Alr1616 in Synechocystis sp. PCC 6803: A Protein Associated with Oxidative Stress Response*
**作者**: Wang, L., et al.
**摘要**: 本研究在大肠杆菌中重组表达了蓝藻(聚球藻PCC 6803)的Alr1616蛋白，并证实其通过硫氧还蛋白依赖机制参与氧化应激保护。实验表明，Alr1616重组蛋白可增强宿主对H₂O₂的抗性，可能与自由基清除相关。
2. **标题**: *Structural Insights into the All1616 Protein from Nostoc sp. PCC 7120: A Novel Thioredoxin-like Fold*
**作者**: Zhang, Y., et al.
**摘要**: 通过X射线晶体学解析了念珠藻PCC 7120来源的All1616重组蛋白的3D结构，发现其具有硫氧还蛋白样折叠结构域。功能分析提示其可能作为电子载体参与光合作用中的电子传递链。
3. **标题**: *Heterologous Expression and Biochemical Analysis of All1616 in Chlamydomonas reinhardtii*
**作者**: Kim, S., & Park, H.
**摘要**: 在莱茵衣藻中异源表达All1616重组蛋白，发现其定位于叶绿体基质。该蛋白在缺氮条件下表达上调，可能通过调节碳代谢通路适应营养胁迫环境。
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**注**：以上文献为模拟示例，实际研究中建议通过 **PubMed** 或 **Web of Science** 以关键词“All1616 recombinant protein”或“Alr1616 gene function”检索最新论文。若需具体文献，可进一步提供研究方向或补充基因背景信息。
**Background of All1616 Recombinant Protein**
The All1616 recombinant protein is derived from the *all1616* gene, originally identified in the cyanobacterium *Synechocystis* sp. PCC 6803. a model organism for studying photosynthesis and stress responses. The *all1616* gene belongs to the "high-light-inducible" (*hli*) gene family, which is upregulated under high-light stress and other environmental challenges. These genes encode small, hydrophobic proteins thought to play roles in photoprotection, likely by stabilizing photosynthetic complexes or dissipating excess energy to prevent oxidative damage.
Recombinant All1616 is produced using genetic engineering techniques, where the *all1616* gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale protein production. The protein is often fused with tags like His-tags for simplified purification via affinity chromatography. Its recombinant form allows researchers to study its structure, function, and interactions in vitro, bypassing challenges associated with its native hydrophobic nature.
Studies suggest All1616 may interact with photosystem II (PSII) or associated pigments, contributing to stress adaptation mechanisms in cyanobacteria. Its recombinant version is instrumental in elucidating its binding partners, structural dynamics, and potential biotechnological applications, such as improving stress tolerance in engineered photosynthetic organisms. Further research aims to clarify its precise role in photoprotection and explore its utility in bioenergy or agricultural systems.
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