| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | wac |
| Uniprot No | P10104 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-487aa |
| 氨基酸序列 | TDIVLNDLPFVDGPPAEGQSRISWIKNGEEILGADTQYGSEGSMNRPTVSVLRNVEVLDKNIGILKTSLETANSDIKTIQGILDVSGDIEALAQIGINKKDISDLKTLTSEHTEILNGTNNTVDSILADIGPFNAEANSVYRTIRNDLLWIKRELGQYTGQDINGLPVVGNPSSGMKHRIINNTDVITSQGIRLSELETKFIESDVGSLTIEVGNLREELGPKPPSFSQNVYSRLNEIDTKQTTVESDISAIKTSIGYPGNNSIITSVNTNTDNIASINLELNQSGGIKQRLTVIETSIGSDDIPSSIKGQIKDNTTSIESLNGIVGENTSSGLRANVSWLNQIVGTDSSGGQPSPPGSLLNRVSTIETSVSGLNNAVQNLQVEIGNNSAGIKGQVVALNTLVNGTNPNGSTVEERGLTNSIKANETNIASVTQEVNTAKGNISSLQGDVQALQEAGYIPEAPRDGQAYVRKDGEWVFLSTFLSPA |
| 预测分子量 | 67.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WAC重组蛋白的3篇代表性文献示例(内容为模拟概括，实际文献需根据具体研究检索)：
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1. **文献名称**：*WAC interacts with p53 and modulates its transcriptional activity in DNA damage response*
**作者**：Olsen JV, et al.
**摘要**：该研究通过重组WAC蛋白的体外实验，发现WAC通过其WW结构域与肿瘤抑制蛋白p53直接结合，增强p53对下游凋亡基因的转录调控能力，揭示了WAC在DNA损伤应答中的潜在作用机制。
2. **文献名称**：*Structural and functional analysis of the WAC protein in transcriptional elongation*
**作者**：Hammet A, et al.
**摘要**：作者利用重组WAC蛋白进行晶体结构解析，发现其C端结构域与RNA聚合酶II的磷酸化CTD区域相互作用，并通过体外转录实验证明WAC通过调控组蛋白修饰酶招募，影响转录延伸效率。
3. **文献名称**：*WAC recruits the nuclear exosome complex to regulate chromatin dynamics*
**作者**：Cottrell KA, et al.
**摘要**：研究通过重组WAC蛋白与核外泌体复合体的共纯化实验，证明WAC作为支架蛋白介导外泌体与染色质重塑复合体(如CoREST)的相互作用，从而参与基因沉默和异染色质形成。
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注：以上文献为示例性概括，实际研究需通过PubMed、Google Scholar等平台检索关键词“WAC protein recombinant”或“WAC gene function”获取。
**Background of WAC Recombinant Protein**
The WAC (WW domain-containing adapter protein with coiled-coil) protein is a multifunctional scaffold protein implicated in diverse cellular processes, including transcriptional regulation, cell cycle control, and autophagy. Initially identified through its interaction with the transcription factor c-Myc, WAC contains structural motifs such as WW domains (protein-protein interaction modules) and a coiled-coil region, enabling it to serve as an adaptor molecule in signaling networks. Its WW domains bind proline-rich motifs in partner proteins, while the coiled-coil domain facilitates oligomerization or interactions with other coiled-coil-containing proteins.
WAC's role in transcriptional regulation is linked to its association with RNA polymerase II and components of the mRNA splicing machinery, suggesting involvement in transcription elongation and RNA processing. Additionally, WAC interacts with the tumor suppressor p53. modulating its stability and activity in response to DNA damage. Studies also highlight its function in autophagy, where WAC promotes autophagosome formation by interacting with autophagy-related proteins like LC3 and GABARAP.
The recombinant WAC protein, generated via genetic engineering in systems like *E. coli* or mammalian cells, retains these functional domains, enabling researchers to study its biochemical interactions, structural properties, and post-translational modifications *in vitro*. Recombinant WAC is particularly valuable for deciphering its roles in diseases; for example, WAC deletions or mutations are associated with neurodevelopmental disorders and cancer. Its dysregulation has been observed in leukemia and glioblastoma, underscoring its potential as a therapeutic target.
Overall, WAC recombinant protein serves as a critical tool for unraveling the molecular mechanisms of cellular homeostasis and disease, bridging gaps between structural biology, signaling pathways, and translational research.
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