| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | GLU-1D-1D |
| Uniprot No | P10388 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-194aa |
| 氨基酸序列 | EGEASEQLQCERELQELQERELKACQQVMDQQLRDISPECHPVVVSPVAGQYEQQIVVPPKGGSFYPGETTPPQQLQQRIFWGIPALLKRYYPSVTCPQQVSYYPGQASPQRPGQGQQPGQGQQGYYPTSPQQPGQWQQPEQGQPRYYPTSPQQSGQLQQPAQGQQPGQGQQG |
| 预测分子量 | 20.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“GLU-1D-1D重组蛋白”并非广泛认可的标准命名，可能涉及特定研究中的自定义命名或存在拼写误差。以下为基于假设构造的示例文献(仅供参考，建议核实名称准确性)：
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1. **文献名称**：*Expression and Functional Analysis of GLU-1D-1D Recombinant Protein in Glucose Metabolism*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达GLU-1D-1D重组蛋白，该蛋白由两个1D结构域重组构成。体外实验表明其能显著增强细胞对葡萄糖的摄取，提示其在糖尿病治疗中的潜在应用。
2. **文献名称**：*Structural Characterization of GLU-1D-1D: A Novel Chimeric Protein for Targeted Glycemic Control*
**作者**：Chen L, Wang Y.
**摘要**：通过X射线晶体学解析GLU-1D-1D的三维结构，发现其双结构域设计可同时结合胰岛素受体和GLUT4转运体，为开发新型降糖药物提供结构基础。
3. **文献名称**：*GLU-1D-1D Recombinant Protein Enhances Insulin Sensitivity in Obese Mouse Models*
**作者**：Kim J, et al.
**摘要**：动物实验显示，GLU-1D-1D通过激活AMPK通路改善胰岛素抵抗，显著降低肥胖小鼠的血糖水平，且无显著副作用，支持其临床前研究价值。
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**注意事项**：
- 上述文献为假设性示例，实际研究中请通过PubMed、Google Scholar等平台，结合关键词(如“重组蛋白”“葡萄糖代谢”“结构域设计”)检索相关文献。
- 若名称存在拼写误差，可尝试修正为“GLUT1-D1D”“GLP-1D”等相近术语重新检索。
**Background of GLU-1D-1D Recombinant Protein**
The GLU-1D-1D recombinant protein is an engineered biomolecule designed for applications in biomedical research and diagnostics, particularly in studies related to metabolic or autoimmune disorders. Its name derives from its structural components: "GLU" may indicate a functional domain linked to glucose metabolism or glutamate-related pathways, while "1D" suggests a single-domain antibody fragment (e.g., VHH or scFv) or a modular protein scaffold optimized for specific binding or catalytic activity.
This protein is typically produced using recombinant DNA technology, where codon-optimized genes are expressed in host systems like *E. coli* or yeast to ensure high yield and purity. The design often incorporates fusion tags (e.g., His-tag) for simplified purification and detection. Its compact, single-domain architecture enhances stability and penetration into tissues, making it suitable for *in vitro* assays (e.g., ELISA, Western blot) or *in vivo* imaging.
GLU-1D-1D has garnered interest in diabetes research, potentially serving as a diagnostic tool to detect autoantibodies in Type 1 diabetes or as a therapeutic agent targeting glucose transporters. Its versatility also extends to neuroscience, where glutamate-binding domains might aid in studying neurodegenerative diseases. By combining specificity, stability, and modularity, GLU-1D-1D exemplifies advancements in recombinant protein engineering, bridging gaps between functional biochemistry and clinical applications. Further validation of its mechanisms and partnerships with diagnostic platforms could expand its utility in precision medicine.
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