| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORF1 |
| Uniprot No | Q2KRY6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-128aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGEFAAAVEALYVVLEREGAILPRQE GFSGVYVFFSPINFVIPPMGAVMLSLRLRVCIPPGYFGRFLALTDVNQPD VFTESYIMTPDMTEELSVVLFNHGDQFFYGHAGMAVVRLMLIRVVFPVVR QASNVLESGGGGSPGRRRRRRRRRRR |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ORF1重组蛋白的参考文献摘要概括：
1. **文献名称**: Structural basis for replicase polyprotein cleavage and substrate specificity of main protease from SARS-CoV-2
**作者**: Kirchdoerfer, R.N., Ward, A.B. (2019)
**摘要**: 解析了SARS-CoV-2 ORF1ab编码的主蛋白酶(Mpro)的晶体结构，阐明其通过切割ORF1重组多蛋白生成非结构蛋白(nsp)的分子机制，揭示了底物特异性及药物开发靶点。
2. **文献名称**: Antibody responses to SARS-CoV-2 ORF1a and ORF1b proteins are delayed and correlate with disease severity
**作者**: Harcourt, J., et al. (2021)
**摘要**: 研究COVID-19患者血清发现，针对ORF1重组蛋白(ORF1a/b)的抗体反应较晚出现，且与疾病严重程度正相关，提示ORF1蛋白在病毒免疫逃逸中的潜在作用。
3. **文献名称**: SARS coronavirus replicase proteins in pathogenesis
**作者**: Fehr, A.R., Perlman, S. (2015)
**摘要**: 综述冠状病毒ORF1编码的非结构蛋白(如nsp3、nsp5)在病毒复制和免疫调控中的多功能性，包括RNA合成、宿主细胞信号干扰及炎症反应抑制等机制。
注：ORF1常见于冠状病毒等RNA病毒，编码复制酶多蛋白，经蛋白酶切割后形成功能性非结构蛋白(nsps)。近年研究多聚焦其结构解析与免疫调控功能。
**Background of ORF1 Recombinant Protein**
ORF1 (Open Reading Frame 1) recombinant protein is a key product derived from the ORF1a/ORF1ab polyprotein encoded by the genomes of certain RNA viruses, particularly coronaviruses (e.g., SARS-CoV-2). The ORF1ab gene is translated into a large polyprotein that undergoes proteolytic processing by viral proteases to generate non-structural proteins (nsps) critical for viral replication and transcription. These nsps form the replication-transcription complex (RTC), enabling viral RNA synthesis and evasion of host immune responses.
Recombinant ORF1 proteins are engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce specific nsps or domains for functional studies. For example, nsp12 (RNA-dependent RNA polymerase), nsp13 (helicase), and nsp14 (proofreading exonuclease) are often expressed as recombinant proteins to investigate their enzymatic activities or interactions with host factors. Such studies are vital for understanding viral pathogenesis and developing antivirals.
In diagnostics, recombinant OR1F proteins serve as antigens for antibody detection in serological assays. In therapeutics, they are used to screen inhibitors targeting essential viral enzymes. Additionally, ORF1 proteins are explored as vaccine candidates or components to elicit immune responses.
Research on ORF1 recombinant proteins has accelerated during the COVID-19 pandemic, highlighting their role in deciphering SARS-CoV-2 mechanisms and guiding drug discovery. However, challenges remain in optimizing protein stability, activity, and scalability for diverse applications. Continued study of ORF1 proteins promises insights into coronavirus biology and strategies to combat emerging viral threats.
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