| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFM1 |
| Uniprot No | P25038 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-676aa |
| 氨基酸序列 | KGKRRNQISKKELKPLNFSIPNYISVNKLANLLNCRVERLIKDLTALGFENITTTYILSKEYVELILQEYNFALPNLSTSTNLDNVYDELKSPVNPKLLTKRAPVVTIMGHVDHGKTTIIDYLRKSSVVAQEHGGITQHIGAFQITAPKSGKKITFLDTPGHAAFLKMRERGANITDIIVLVVSVEDSLMPQTLEAIKHAKNSGNEMIIAITKIDRIPQPKEREKKIEKVINDLIVQGIPVEKIGGDVQVIPISAKTGENMDLLEESIVLLSEVMDIRAENSPKTIAEGWIIESQVKKQVGNVATVLVKKGTLQKGKILICGNTFCKIKNLIDDKGIPILKATPSYATEVLGWKDVPHVGDEVIQVKSEAIAKKFISKRQDLIEVQKNSSIVEKLNEERALAKEQHLNKELEHENTVQEHEQNTGPKLINYIIKCDVSGSAEAVSESISSLGNDEVRCNVISSSVGIPTESDLKMAQITESTILCFNLGNLPSEVINNRAGIKIKQYNVIYKLIEDVTETLTENLKPIFEKKIVSTVDVRETFDFRLKKKIIRIAGCKVNNGVIKKNSLVQVVRGPNEDVIFDGKISTLKHNKDDVAEVSKGHECGITFESGFEGFKPGDKILVYENVRVPRYL |
| 预测分子量 | 97.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFM1重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献可能需要根据具体研究领域进一步检索)：
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1. **《Expression and purification of recombinant IFM1 in Escherichia coli for functional studies》**
*作者：Smith J, Lee K, et al.*
**摘要**：本研究报道了通过大肠杆菌表达系统成功克隆并纯化IFM1重组蛋白。实验优化了诱导条件以提高可溶性蛋白产量，并验证了重组IFM1的酶活性，为后续功能研究奠定基础。
2. **《Structural characterization of IFM1 using X-ray crystallography》**
*作者：Zhang Y, Wang H, et al.*
**摘要**：通过X射线晶体学解析了IFM1重组蛋白的三维结构，揭示了其活性位点的关键氨基酸残基。研究为理解IFM1在细胞色素c成熟中的作用机制提供了结构依据。
3. **《High-yield production of IFM1 in Pichia pastoris for industrial applications》**
*作者：Garcia R, Müller T, et al.*
**摘要**：利用毕赤酵母表达系统实现IFM1重组蛋白的高效分泌表达，优化了发酵条件，获得高纯度产物。该研究展示了IFM1在生物技术工业中的应用潜力。
4. **《Functional analysis of IFM1 in mitochondrial apoptosis pathways》**
*作者：Chen L, Tanaka M, et al.*
**摘要**：通过基因敲除和重组IFM1回补实验，证明IFM1通过调控细胞色素c的成熟参与线粒体凋亡通路，为相关疾病治疗提供了新靶点。
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如需具体文献，建议在PubMed、Web of Science等数据库中使用关键词“IFM1 recombinant protein”或“IFM1 gene expression”进一步检索。
IFM1 (Inner membrane fusion protein 1) is a mitochondrial protein critical for maintaining mitochondrial dynamics and structural integrity. It plays a key role in regulating the fusion of the mitochondrial inner membrane, a process essential for mitochondrial network organization, energy production, and cellular homeostasis. IFM1 is functionally conserved across eukaryotes, with homologs like mammalian OPA1 and yeast Mgm1 sharing similar roles in inner membrane remodeling. Dysregulation of IFM1-related pathways has been linked to mitochondrial disorders, neurodegenerative diseases, and aging.
Recombinant IFM1 protein is produced through heterologous expression systems (typically E. coli or yeast) to enable biochemical and functional studies. The recombinant form retains conserved structural features including a GTPase domain critical for membrane fusion activity and hydrophobic regions mediating membrane association. Researchers utilize purified IFM1 to investigate its interaction with phospholipids, regulatory proteins (e.g., prohibitins), and metabolic signals. Its recombinant version also serves as an antigen for antibody development and a tool for screening potential modulators of mitochondrial fusion. Recent studies employ IFM1 in structural biology (cryo-EM analyses) and drug discovery platforms targeting mitochondrial dysfunction. The protein's temperature-sensitive conformational changes and pH-dependent oligomerization properties make it particularly interesting for mechanistic studies of mitochondrial dynamics.
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