| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ompD |
| Uniprot No | P37592 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-362aa |
| 氨基酸序列 | AEVYNKDGNKLDLYGKVHAQHYFSDDNGSDGDKTYARLGFKGETQINDQLTGFGQWEYEFKGNRTESQGADKDKTRLAFAGLKFADYGSFDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNTDFFGLVEGLNFAAQYQGKNDRDGAYESNGDGFGLSATYEYEGFGVGAAYAKSDRTNNQVKAASNLNAAGKNAEVWAAGLKYDANNIYLATTYSETLNMTTFGEDAAGDAFIANKTQNFEAVAQYQFDFGLRPSIAYLKSKGKNLGTYGDQDLVEYIDVGATYYFNKNMSTFVDYKINLLDDSDFTKAAKVSTDNIVAVGLNYQF |
| 预测分子量 | 53.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OmpD重组蛋白的3篇参考文献摘要概括(注：文献信息为示例，实际需根据具体文献调整)：
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1. **文献名称**：*Cloning, Expression, and Purification of Salmonella Typhimurium OmpD Recombinant Protein*
**作者**：Zhang et al.
**摘要**：研究通过PCR扩增沙门氏菌ompD基因，构建至pET载体，在大肠杆菌BL21中诱导表达。利用镍柱亲和层析纯化获得高纯度重组OmpD蛋白，并验证其与抗体的结合活性，为后续疫苗开发提供基础。
2. **文献名称**：*Immunogenicity of Recombinant OmpD in a Mouse Model of Salmonella Infection*
**作者**：Fernández et al.
**摘要**：评估重组OmpD蛋白作为亚单位疫苗的潜力。实验显示，免疫小鼠后能诱导显著Th1型免疫反应，提高对沙门氏菌攻击的存活率，表明OmpD在抗感染中具有保护性抗原功能。
3. **文献名称**：*Optimization of Recombinant OmpD Expression in E. coli Using Response Surface Methodology*
**作者**：Wang & Li
**摘要**：通过响应面法优化OmpD重组蛋白的表达条件(如温度、IPTG浓度)，最终使蛋白产量提升2.3倍。SDS-PAGE和Western blot证实蛋白正确表达，适用于大规模生产。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“recombinant OmpD protein”或“Salmonella OmpD expression”获取最新研究。
**Background of ompD Recombinant Protein**
OmpD, a major outer membrane protein (OMP) predominantly found in *Salmonella* species and other Gram-negative bacteria, plays a critical role in bacterial physiology and host-pathogen interactions. As a porin, it forms β-barrel channels in the outer membrane, facilitating the passive diffusion of small hydrophilic molecules, including nutrients and ions. Its expression is influenced by environmental factors, such as osmolarity and antimicrobial stress, suggesting its adaptability in diverse niches.
The recombinant ompD protein is engineered through cloning the *ompD* gene into expression vectors (e.g., *E. coli* or yeast systems) for large-scale production. This approach enables the study of ompD’s structural and functional properties in controlled settings. Recombinant ompD retains its antigenic epitopes, making it valuable for immunological studies. For instance, it has been explored as a vaccine candidate due to its surface exposure and role in bacterial survival. Studies in *Salmonella* highlight its potential to elicit protective immune responses, though efficacy varies across serovars.
Additionally, recombinant ompD serves as a tool for diagnostic applications. Antibodies against ompD can detect bacterial infections, aiding in rapid pathogen identification. Its role in antibiotic resistance is also under investigation, as altered porin expression may reduce drug permeability.
Despite its utility, challenges persist, including maintaining protein stability during purification and ensuring proper folding to mimic native conformations. Advances in recombinant technology and structural biology continue to refine ompD production, enhancing its applicability in therapeutic and biotechnological contexts. Overall, ompD recombinant protein bridges fundamental research and translational solutions in combating bacterial infections.
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