| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | eaeA |
| Uniprot No | P19809 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-939aa |
| 氨基酸序列 | MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENYFKLGSDSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNIPHDINGTERSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNVYKVTARAYDRNGNSSNNVLLTITVLSNGQVVDQVGVTDFTADKTSAKADGTEAITYTATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVTLKSDKPGQVVVSAKTAEMTSALNANAVIFVDQTKASITEIKADKTTAVANGQDAITYTVKVMKGDKPVSNQEVTFTTTLGKLSNSTEKTDTNGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFFTTLTIDDGNIEIVGTGVKGKLPTVWLQYGQVNLKASGGNGKYTWRSANPAIASVDASSGQVTLKEKGTTTISVISSDNQTATYTIATPNSLIVPNMSKRVTYNDAVNTCKNFGGKLPSSQNELENVFKAWGAANKYEYYKSSQTIISWVQQTAQDAKSGVASTYDLVKQNPLNNIKASESNAYATCVK |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于eaeA重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要内容概括：
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1. **标题**：*"Cloning and Expression of the eaeA Gene Encoding Intimin in Enterohemorrhagic E. coli"*
**作者**：Smith J, et al.
**摘要**：本研究成功克隆了EHEC O157:H7的eaeA基因，并在大肠杆菌表达系统中生成重组Intimin蛋白。通过His标签纯化获得高纯度蛋白，证实其与宿主细胞受体Tir的特异性结合，为后续致病机制研究奠定基础。
2. **标题**：*"Recombinant Intimin as a Vaccine Candidate Against EPEC Infection"*
**作者**：Wang L, et al.
**摘要**：评估重组eaeA蛋白(Intimin)的免疫保护效果。实验显示，接种该蛋白的小鼠产生高水平IgG抗体，并在攻毒试验中显著降低细菌定植，提示其作为亚单位疫苗的潜力。
3. **标题**：*"Development of an eaeA-Based ELISA for Diagnosing Enteropathogenic E. coli Infections"*
**作者**：Garcia R, et al.
**摘要**：利用重组eaeA蛋白作为抗原建立ELISA检测法，验证其在临床样本中诊断EPEC感染的敏感性和特异性。结果显示，该方法与PCR检测结果高度一致，可作为快速诊断工具。
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**注**：以上文献为假设性示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献。建议结合关键词“eaeA recombinant protein”、“Intimin expression”及“EPEC/EHEC diagnosis”进一步查找。
**Background of eaeA Recombinant Protein**
The *eaeA* gene encodes intimin, a critical virulence factor in enteropathogenic *Escherichia coli* (EPEC) and enterohemorrhagic *E. coli* (EHEC). These pathogens cause severe gastrointestinal diseases, particularly in children and immunocompromised individuals. Intimin facilitates bacterial adherence to host intestinal epithelial cells by binding to its receptor, Tir (translocated intimin receptor), which is secreted by the bacteria itself via a type III secretion system. This interaction leads to the formation of characteristic "attaching and effacing" (A/E) lesions, disrupting intestinal integrity and promoting infection.
Recombinant eaeA protein is produced through genetic engineering, typically by cloning the *eaeA* gene into expression vectors (e.g., *E. coli* or yeast systems) and purifying the expressed protein. Studying this recombinant protein has been pivotal in understanding bacterial pathogenesis, host-pathogen interactions, and the structural basis of intimin-Tir binding. Its extracellular domain, particularly the C-terminal region, exhibits variability across intimin subtypes (e.g., α, β, γ), influencing host and tissue specificity.
Applications of eaeA recombinant protein include diagnostic tool development (e.g., ELISA-based detection of EHEC/EPEC), vaccine design, and mechanistic studies exploring adhesion inhibitors. Structural analyses (e.g., X-ray crystallography) using the recombinant protein have revealed key binding motifs, aiding in the design of anti-adhesion therapies. Furthermore, it serves as an antigen in immunological studies to evaluate host antibody responses.
Overall, eaeA recombinant protein is a vital resource for advancing research on diarrheal pathogens, therapeutic development, and understanding microbial adhesion mechanisms critical to infection.
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