| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | cssB |
| Uniprot No | P53510 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-167aa |
| 氨基酸序列 | GNWQYKSLDVNVNIEQNFIPDIDSAVRIIPVNYDSDPKLNSQLYTVEMTIPAGVSAVKIVPTDSLTSSGQQIGKLVNVNNPDQNMNYYIRKDSGAGKFMAGQKGSFSVKENTSYTFSAIYTGGEYPNSGYSSGTYAGHLTVSFYSN |
| 预测分子量 | 31.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSSB重组蛋白的假设性参考文献示例(请注意，文献名称及作者为虚构，实际研究需根据具体术语核实)：
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1. **文献名称**：*Cloning and Functional Analysis of Recombinant CSSB Protein in Bacillus subtilis*
**作者**：Saucedo-Ramírez, L., et al. (2018)
**摘要**：研究报道了CSSB基因的克隆及其在大肠杆菌中的重组表达，分析了CSSB蛋白在细菌孢子形成中的结构功能，证实其参与孢子衣层的组装过程。
2. **文献名称**：*Immunogenicity of Recombinant CSSB as a Vaccine Carrier*
**作者**：Zhang, Y., et al. (2020)
**摘要**：探讨了重组CSSB蛋白作为疫苗载体的潜力，通过动物实验证明其可诱导特异性抗体反应，为新型疫苗开发提供理论依据。
3. **文献名称**：*Optimized Purification of Recombinant CSSB for Biotechnological Applications*
**作者**：Chen, H., et al. (2016)
**摘要**：开发了一种高效纯化重组CSSB蛋白的方法，结合亲和层析与离子交换技术，获得高纯度蛋白并验证其体外稳定性。
4. **文献名称**：*Structural Insights into CSSB Protein via Cryo-EM*
**作者**：Kim, J., et al. (2021)
**摘要**：利用冷冻电镜解析了重组CSSB蛋白的三维结构，揭示了其与辅因子结合的分子机制，为靶向药物设计提供结构基础。
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**注意**：以上内容为示例，实际文献需根据**CSSB蛋白的正确拼写及研究领域**(如孢子形成、结构生物学等)在学术数据库(PubMed、Google Scholar等)中检索。建议确认术语准确性(如是否为CsSB、CotB等类似名称)以获取真实研究。
**Background of CSSB Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, have revolutionized biomedical research and therapeutic development. Among these, CSSB (Cell Signaling and Structural Biology) recombinant proteins represent a specialized class designed to study cellular signaling pathways and protein structures with high precision. Developed to address challenges in protein stability, solubility, and functional accuracy, CSSB recombinant proteins are typically produced using advanced expression systems, such as *E. coli*, yeast, or mammalian cells, followed by stringent purification processes.
The design of CSSB proteins often incorporates tags (e.g., His-tags, GST-tags) for efficient purification and detection, while mutations or modifications may be introduced to enhance conformational stability or mimic post-translational modifications. These proteins are critical tools for elucidating molecular mechanisms in diseases like cancer, neurodegenerative disorders, and immune dysregulation. For instance, CSSB-engineered cytokines, kinases, or receptors enable researchers to dissect signaling cascades or screen drug candidates targeting specific pathways.
Additionally, CSSB recombinant proteins underpin structural biology efforts, facilitating X-ray crystallography and cryo-EM studies to resolve atomic-level details of protein interactions. Their applications extend to diagnostics, where they serve as antigens or standards in assays, and to therapeutics, including monoclonal antibodies and enzyme replacement therapies.
The development of CSSB recombinant proteins reflects advancements in synthetic biology and bioprocessing, emphasizing reproducibility and scalability to meet research and clinical demands. As personalized medicine grows, these proteins are poised to play a pivotal role in tailoring treatments based on individual molecular profiles.
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