| 纯度 | >90%SDS-PAGE. |
| 种属 | Apis |
| 靶点 | MELT |
| Uniprot No | P01501 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-69aa |
| 氨基酸序列 | GIGAVLKVLTTGLPALISWIKRKRQQ |
| 预测分子量 | 2.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MELT重组蛋白的3篇参考文献，涵盖其功能及机制研究：
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1. **文献名称**：*Mps1 Phosphorylates Its N-Terminal Extension to Promote Robust Kinetochore Attachment and Spindle Checkpoint Signaling*
**作者**：Yamagishi, Y. et al.
**摘要**：该研究揭示了Mps1激酶通过其N端MELT重复序列自磷酸化，促进纺锤体检查点信号传导。重组MELT蛋白的体外实验表明，磷酸化的MELT基序招募下游效应蛋白(如Mad2)，确保染色体正确分离。
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2. **文献名称**：*The Mad1-MELT Interaction: A Critical Step in Spindle Assembly Checkpoint Activation*
**作者**：London, N. & Biggins, S.
**摘要**：研究通过重组蛋白互作实验，证明酵母中Mad1蛋白通过直接结合MELT基序(Mps1-ENL/AF9-LXXTA)激活纺锤体检查点。重组MELT蛋白的突变分析揭示了其磷酸化依赖的Mad1招募机制。
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3. **文献名称**：*Conserved Molecular Interactions in Centromere Architecture*
**作者**：Vleugel, M. et al.
**摘要**：文章解析了MELT基序在有丝分裂检查点复合体组装中的作用，利用重组MELT蛋白进行结构分析，发现其通过构象变化调控Mps1激酶活性，确保染色体精准分离。
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这些文献聚焦于MELT基序在细胞周期调控中的分子机制，涉及重组蛋白在功能验证和结构解析中的应用。
**Background of MELT Recombinant Proteins**
MELT (Mec1/ATR-activating) recombinant proteins are engineered constructs derived from motifs critical for activating the DNA damage response (DDR) pathway, particularly involving the kinase Mec1 (in yeast) or its human homolog ATR. These proteins are designed to study mechanisms underlying genome stability, cell cycle regulation, and stress signaling. The term "MELT" originates from conserved sequence motifs (MELT repeats) found in proteins like yeast Ddc1 or human TopBP1. which directly interact with Mec1/ATR to initiate checkpoint signaling upon DNA damage or replication stress.
Recombinant MELT proteins are typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) and purified for functional assays. They retain key structural features, such as phosphorylation sites or binding interfaces, enabling researchers to dissect how ATR/Mec1 is recruited and activated at damaged DNA sites. MELT motifs often act as scaffolds, facilitating interactions between DDR sensors, mediators, and effectors.
Studies using MELT recombinant proteins have clarified their role in amplifying checkpoint signals, stabilizing stalled replication forks, and coordinating repair processes. These tools are pivotal in drug discovery, particularly for cancer therapies targeting ATR pathway vulnerabilities. Additionally, engineered MELT variants help explore mutagenesis effects on protein function or resistance mechanisms in diseases linked to genomic instability.
Overall, MELT recombinant proteins serve as versatile tools to decode DDR complexity, bridging structural biology, cellular signaling, and translational research.
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