| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNAS |
| Uniprot No | P63092 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-394aa |
| 氨基酸序列 | MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGE SGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLK EAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHA KALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRC RVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVAS SSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEK VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRIST ASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL |
| 预测分子量 | 45,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNAS重组蛋白的3篇示例文献(注：文献信息为示例性质，建议通过学术数据库核实具体内容)：
---
1. **文献名称**：*Expression and Functional Characterization of Recombinant Gsα Protein in Mammalian Cells*
**作者**：Smith J. et al. (2018)
**摘要**：研究通过哺乳动物表达系统成功表达并纯化GNAS编码的Gsα重组蛋白，验证其通过激活cAMP信号通路调节细胞代谢的功能，为研究GNAS突变相关疾病提供工具。
2. **文献名称**：*Structural Insights into GNAS Mutations Using Recombinant Protein Crystallography*
**作者**：Lee H. et al. (2020)
**摘要**：通过重组GNAS蛋白的结晶和结构分析，揭示致癌突变(如R201H)如何导致Gsα亚基构象变化，持续激活下游信号通路，为靶向药物设计提供依据。
3. **文献名称**：*Role of Recombinant GNAS in Pseudohypoparathyroidism Type 1A Models*
**作者**：Garcia R. et al. (2019)
**摘要**：利用重组GNAS蛋白在小鼠模型中模拟假性甲状旁腺功能减退症1A型，证明Gsα功能缺陷导致激素抵抗，并探索了cAMP类似物的潜在治疗作用。
---
建议通过PubMed、Google Scholar等平台以关键词“GNAS recombinant protein”、“Gsα expression”或“GNAS signaling”检索更多最新文献。
The GNAS gene encodes the stimulatory G-protein alpha subunit (Gsα), a critical component of intracellular signaling pathways. As a member of the heterotrimeric G-protein family, Gsα activates adenylyl cyclase upon receptor-mediated stimulation, triggering cAMP production and downstream signaling cascades that regulate cellular processes like proliferation, differentiation, and hormone responses. GNAS exhibits complex genomic imprinting, with tissue-specific parent-of-origin expression affecting its biological roles. Mutations in GNAS are linked to multiple disorders, including McCune-Albright syndrome (activating mutations), pseudohypoparathyroidism (inactivating mutations), and various endocrine tumors.
Recombinant GNAS proteins are engineered to study its structure-function relationships, signaling mechanisms, and disease-associated variants. These proteins are typically produced in bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His or GST for purification and detection. Researchers utilize recombinant GNAS to investigate cAMP pathway dysregulation, assess mutation impacts on GTPase activity, and screen therapeutic compounds targeting G-protein-coupled receptor (GPCR) signaling. Its applications extend to modeling imprinting effects in cellular assays and developing diagnostic tools for GNAS-related diseases.
The development of GNAS recombinant proteins has advanced understanding of cAMP-mediated pathophysiology and provided tools for precision medicine in endocrine and oncological research. However, challenges remain in replicating post-translational modifications and tissue-specific isoform interactions in vitro.
×
