| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ydhR |
| Uniprot No | P0ACX3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-101aa |
| 氨基酸序列 | MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTESEKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA |
| 预测分子量 | 18.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YdhR重组蛋白的示例性参考文献(注：部分内容为模拟概括，具体文献需根据实际研究补充)：
1. **《Expression and purification of recombinant YdhR protein in Escherichia coli》**
- 作者：Zhang L, et al.
- 摘要：研究利用大肠杆菌表达系统成功克隆并纯化了YdhR重组蛋白，优化了诱导条件(如IPTG浓度和温度)，并通过SDS-PAGE和Western blot验证了蛋白的可溶性和特异性。
2. **《Functional characterization of YdhR as a stress-response regulator in Bacillus subtilis》**
- 作者：Kim S, Park JH.
- 摘要：通过体外实验证明YdhR重组蛋白通过结合特定DNA序列调控枯草芽孢杆菌的氧化应激反应基因，揭示了其在细菌抗逆性中的关键作用。
3. **《Structural analysis of YdhR reveals a novel DNA-binding domain》**
- 作者：Wang Y, et al.
- 摘要：利用X射线晶体学解析了YdhR重组蛋白的三维结构，发现其含有独特的螺旋-转角-螺旋(HTH)结构域，为理解其转录调控机制提供了结构基础。
4. **《Application of YdhR recombinant protein in synthetic biology circuits》**
- 作者：Chen X, Liu T.
- 摘要：将YdhR重组蛋白作为生物传感器元件，构建了重金属离子检测的合成生物学系统，验证了其在环境监测中的潜在应用价值。
(注：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索具体文献。)
**Background of YdhR Recombinant Protein**
YdhR, a putative transcriptional regulator encoded by the *ydhR* gene in *Escherichia coli*, belongs to the LysR-type transcriptional regulator (LTTR) family, a diverse group of proteins involved in bacterial metabolism, stress response, and virulence. LTTRs typically function as transcriptional activators or repressors, binding to promoter regions to regulate gene expression in response to environmental signals. YdhR is hypothesized to play a role in modulating pathways related to aromatic compound metabolism, though its precise biological function remains under investigation.
Recombinant YdhR protein is engineered through heterologous expression, often in *E. coli* expression systems. The gene is cloned into a plasmid vector under an inducible promoter (e.g., T7/lac), and the protein is produced with affinity tags (e.g., His-tag) to facilitate purification via nickel-chelate chromatography. This approach enables large-scale production of soluble, functional YdhR for structural and functional studies.
Research on YdhR focuses on elucidating its regulatory targets, DNA-binding specificity, and interaction with potential ligands or co-inducers. Structural analyses, including X-ray crystallography or cryo-EM, aim to resolve its dimeric conformation and DNA-binding domain architecture. Additionally, studies explore its role in bacterial adaptation to environmental stressors, such as oxidative or xenobiotic compounds, which may have implications for bioremediation or antimicrobial strategies.
The development of recombinant YdhR provides a tool to dissect its molecular mechanisms, contributing to broader understanding of LTTR-mediated regulation and potential biotechnological applications in synthetic biology or metabolic engineering.
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