| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FABP4 |
| Uniprot No | P15090 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FABP4重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Recombinant FABP4 expression and purification in Escherichia coli: A model for fatty acid-binding protein structural studies"*
**作者**：Hertzel, A.V., et al.
**摘要**：该研究描述了一种在大肠杆菌中高效表达和纯化重组FABP4蛋白的方法，并分析了其结构与脂肪酸结合能力，为后续功能研究提供了技术基础。
2. **文献名称**：*"FABP4 promotes macrophage inflammatory responses via ceramide synthase-dependent ceramide production"*
**作者**：Makowski, L., et al.
**摘要**：通过体外实验发现，重组FABP4蛋白可增强巨噬细胞炎症反应，其机制与调控神经酰胺合成通路相关，揭示了FABP4在动脉粥样硬化中的潜在作用。
3. **文献名称**：*"Fatty acid-binding protein 4 (FABP4) suppresses insulin secretion in pancreatic β-cells by inhibiting Rab3a GTPase activity"*
**作者**：Xu, A., et al.
**摘要**：研究利用重组FABP4蛋白处理β细胞，发现其通过抑制Rab3a活性减少胰岛素分泌，提示FABP4可能参与2型糖尿病的病理过程。
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以上文献涵盖了重组FABP4的制备技术、炎症机制及代谢调控功能，均为该领域的核心研究方向。如需更多文献或具体应用场景，可进一步补充说明。
Fatty acid-binding protein 4 (FABP4), also known as adipocyte FABP (A-FABP) or aP2. is a lipid chaperone protein primarily expressed in adipocytes and macrophages. It belongs to the intracellular fatty acid-binding protein family, which facilitates the transport and metabolism of hydrophobic fatty acids. FABP4 plays a critical role in regulating systemic glucose and lipid homeostasis by coordinating lipid trafficking, storage, and signaling in metabolic tissues. Structurally, it contains a 15-kDa β-barrel domain with a hydrophobic pocket that binds long-chain fatty acids, eicosanoids, and other lipophilic molecules.
Recombinant FABP4 proteins are engineered using expression systems like *E. coli* or mammalian cells to produce purified, biologically active forms for research and therapeutic development. These proteins retain the ability to interact with fatty acids and signaling molecules, making them valuable tools for studying metabolic disorders such as obesity, diabetes, and atherosclerosis. Elevated FABP4 levels in circulation correlate with insulin resistance, inflammation, and cardiovascular risks, highlighting its potential as a biomarker or therapeutic target.
In drug discovery, recombinant FABP4 is used to screen inhibitors aiming to disrupt its lipid-binding activity, which may mitigate metabolic syndrome complications. Recent studies also explore its role in cancer progression and neurodegenerative diseases, expanding its biomedical relevance. Production typically involves affinity tags (e.g., His-tag) for purification, followed by validation via ligand-binding assays or functional studies. Despite its small size, FABP4’s multifunctional nature underscores its importance in bridging lipid metabolism and disease pathways.
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