| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FABP5 |
| Uniprot No | Q01469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-135aa |
| 氨基酸序列 | ATVQQLEGRWRLVDSKGFDEYMKELGVGIALRKMGAMAKPDCIITCDGKN LTIKTESTLKTTQFSCTLGEKFEETTADGRKTQTVCNFTDGALVQHQEWD GKESTITRKLKDGKLVVECVMNNVTCTRIYEKVE |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FABP5重组蛋白的文献概览(人工模拟非真实文献，仅供示例参考)：
1. **"Expression and purification of recombinant FABP5 in E. coli for ligand-binding studies"**
*作者：Chen L, et al.*
摘要：报道在大肠杆菌系统中高效表达可溶性FABP5重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其与脂肪酸配体的结合活性。
2. **"Structural insights into FABP5 mechanism by X-ray crystallography of recombinant protein"**
*作者：Wang Y, et al.*
摘要：利用重组表达的FABP5蛋白进行结晶和X射线衍射分析，揭示其结合口袋的三维结构特征，探讨其与视黄酸相互作用的分子基础。
3. **"Recombinant FABP5 promotes cancer cell proliferation via PPARβ/δ signaling pathway"**
*作者：Kim S, et al.*
摘要：通过体外实验证明重组FABP5蛋白可增强肿瘤细胞的增殖能力，机制研究显示其通过激活PPARβ/δ信号通路促进细胞周期进程。
(注：以上文献为虚构示例，实际文献需通过PubMed/Google Scholar等平台检索)
Fatty acid-binding protein 5 (FABP5), also known as epidermal-type FABP or keratinocyte-type FABP, is a 15 kDa intracellular lipid chaperone involved in fatty acid transport, metabolism, and signaling. It belongs to the FABP family, which comprises tissue-specific isoforms regulating lipid homeostasis. FABP5 is highly expressed in adipocytes, neurons, skin keratinocytes, and immune cells, where it facilitates the uptake and intracellular trafficking of hydrophobic ligands such as long-chain fatty acids, retinoic acid, and endocannabinoids. Its function is closely tied to cellular processes including inflammation, insulin sensitivity, and cell proliferation. Dysregulation of FABP5 has been implicated in metabolic disorders, neurodegenerative diseases, psoriasis, and cancer progression, particularly in tumorigenesis linked to lipid metabolism reprogramming.
Recombinant FABP5 protein is produced using genetic engineering techniques, often in bacterial (e.g., E. coli) or mammalian expression systems, to yield purified, functional protein for research applications. The recombinant form retains the native structure required for ligand binding, confirmed by circular dichroism or nuclear magnetic resonance (NMR) studies. Production typically involves affinity chromatography purification with tags like His-tag, followed by tag removal and rigorous quality control (e.g., SDS-PAGE, Western blot, mass spectrometry) to ensure homogeneity and bioactivity.
This tool enables mechanistic studies of FABP5-ligand interactions, screening of potential inhibitors for therapeutic development, and structural analyses. It is widely used in lipid metabolism research, cancer biology (notably in tumors overexpressing FABP5), and drug discovery pipelines targeting metabolic or inflammatory pathways. Its standardized availability accelerates studies on lipid-mediated signaling and validation of FABP5 as a biomarker or therapeutic target.
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