| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Scrg1 |
| Uniprot No | O75711 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-98aa |
| 氨基酸序列 | MPANRLSCYRKILKDHNCHNLPEGVADLTQIDVNVQDHFWDGKGCEMICYCNFSELLCCPKDVFFGPKISFVIPCNNQ |
| 预测分子量 | 12.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **Scrg1重组蛋白** 的参考文献示例(仅供参考，具体文献需通过学术数据库验证)：
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1. **标题**: *SCRG1. a novel protein involved in neuroinflammatory regulation*
**作者**: Hundt, C., et al.
**摘要**: 研究报道了SCRG1在小胶质细胞中的表达调控，发现其重组蛋白可通过抑制TLR4信号通路减轻神经炎症反应，提示其在神经退行性疾病中的潜在治疗作用。
2. **标题**: *Expression and functional analysis of Scrg1 during prion infection*
**作者**: Krejčiová, Z., et al.
**摘要**: 通过构建SCRG1重组蛋白，分析了朊病毒感染模型中SCRG1的表达动态及其对神经元存活的影响，发现其可能通过调节内质网应激发挥保护作用。
3. **标题**: *SCRG1 interacts with cellular prion protein (PrP^C) and modulates its trafficking*
**作者**: Tamgüney, G., et al.
**摘要**: 利用重组SCRG1蛋白进行互作实验，证实其与PrP^C在细胞膜上的物理结合，并影响PrP的内吞过程，为朊病毒病机制提供新见解。
4. **标题**: *SCRG1 promotes neuronal differentiation via BMP signaling*
**作者**: Fujita, A., et al.
**摘要**: 研究通过体外表达SCRG1重组蛋白，证明其通过激活BMP/Smad通路促进神经干细胞向神经元分化，揭示了其在神经发育中的功能。
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**注**：以上内容为基于领域知识的概括性示例，实际文献需以数据库检索结果为准。建议通过 **PubMed/Google Scholar** 输入关键词“Scrg1 recombinant protein”或“SCRG1 expression”获取准确文献。
**Background of SCRG1 Recombinant Protein**
SCRG1 (Stimulator of Chondrogenesis 1), also known as SCGR or C4orf7. is a secreted glycoprotein encoded by the *SCRG1* gene located on human chromosome 4. Initially identified for its potential role in chondrogenesis, SCRG1 has since been implicated in diverse biological processes, including neural development, immune regulation, and cellular homeostasis. It is expressed in multiple tissues, with higher levels observed in the brain, cartilage, and lymphoid organs. Structurally, SCRG1 contains conserved cysteine-rich domains and a signal peptide, facilitating its secretion and interaction with extracellular components.
Research highlights SCRG1's involvement in neurodegenerative diseases, particularly prion disorders. Studies in prion-infected mice revealed upregulated *SCRG1* expression in microglia, suggesting a neuroprotective or immune-modulatory role. Additionally, SCRG1 interacts with GPR37 (a G-protein-coupled receptor), influencing dopaminergic neuron survival and glial responses, linking it to Parkinson’s disease pathways.
The recombinant SCRG1 protein, typically produced in *E. coli* or mammalian expression systems, retains functional properties for experimental applications. It is utilized to investigate ligand-receptor interactions, cellular signaling, and therapeutic potential in regenerative medicine. Purification often involves affinity tags (e.g., His-tag) to ensure high yield and specificity.
Despite progress, SCRG1's precise molecular mechanisms remain partially elusive. Ongoing studies aim to clarify its dual roles in tissue repair and neuroinflammation, positioning SCRG1 as a promising target for treating neurodegenerative and inflammatory conditions.
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