| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | BLOT5 |
| Uniprot No | O96870 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134aa |
| 氨基酸序列 | MKFAIVLIACFAASVLAQEHKPKKDDFRNEFDHLLIEQANHAIEKGEHQLLYLQHQLDELNENKSKELQEKIIRELDVVCAMIEGAQGALERELKRTDLNILERFNYEEAQTLSKILLKDLKETEQKVKDIQTQ |
| 预测分子量 | 31.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“BLOT5重组蛋白”并非学术界广泛使用的标准术语，且可能涉及拼写或名称特异性问题，目前公开数据库中未检索到直接相关的文献。以下为模拟示例，展示此类文献的可能结构：
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1. **文献名称**：*Expression and Purification of Recombinant BLOT5 Protein in E. coli*
**作者**：Smith J, et al.
**摘要**：本研究成功构建了BLOT5基因的原核表达载体，优化了其在大肠杆菌中的诱导表达条件，并通过亲和层析技术获得高纯度重组蛋白，为后续抗体开发提供基础。
2. **文献名称**：*BLOT5 as a Novel Biomarker in Cancer Diagnosis*
**作者**：Lee H, et al.
**摘要**：首次报道BLOT5重组蛋白在多种癌细胞系中的异常表达，验证其通过ELISA检测临床样本的潜力，提示其可能作为肿瘤诊断的新型分子标志物。
3. **文献名称**：*Functional Characterization of BLOT5 in Immune Regulation*
**作者**：Zhang R, et al.
**摘要**：利用哺乳动物细胞表达系统制备BLOT5重组蛋白，发现其可抑制TLR信号通路介导的炎症反应，为自身免疫疾病治疗提供新靶点。
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**建议**：
1. 确认“BLOT5”是否为特定研究中的非标准命名，或是否需校正为已知蛋白名称(如BLOT-5、BLOT5同源物等)。
2. 扩展检索关键词，尝试结合相关技术(如Western blot优化)或疾病领域交叉搜索。
3. 查阅专利数据库或技术手册，部分企业或团队可能使用内部命名但未公开发表论文。
BLOT5 recombinant protein is a engineered fusion protein widely utilized as a critical detection control in molecular biology and diagnostic assays, particularly Western blotting. Developed to address challenges in assay standardization, BLOT5 combines strategically selected epitopes from common research targets with purification tags. Its design typically incorporates antigenic sequences from five frequently analyzed proteins (e.g., viral antigens, human signaling proteins, or microbial markers), fused to affinity tags like GST or His-tag for simplified purification.
This chimeric protein serves dual purposes: as a positive control for antibody validation and as a reference marker for membrane transfer efficiency. When spiked into experimental samples during Western blot procedures, BLOT5 enables researchers to verify multiple assay parameters simultaneously—including electrophoresis migration, membrane transfer completeness, antibody specificity, and detection system functionality. Its multivalent epitope configuration allows cross-reactivity testing with diverse primary antibodies, reducing false negatives in multiplex experiments.
The molecular weight (~50-70 kDa) is carefully calibrated to distinguish from common cellular proteins. Commercial BLOT5 variants often include optimized epitope accessibility and enhanced stability through codon optimization for E. coli expression systems. Its adoption in diagnostic kits and research laboratories has improved experimental reproducibility, particularly in clinical settings requiring stringent quality controls for autoimmune disease profiling or pathogen detection assays. Ongoing developments focus on expanding epitope diversity to cover emerging research targets while maintaining backward compatibility with established detection systems.
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