| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FABP9 |
| Uniprot No | Q0Z7S8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMVEPFLGTWKLVSSENFEDYMKELGV NFAARNMAGLVKPTVTISVDGKMMTIRTESSFQDTKISFKLGEEFDETTA DNRKVKSTITLENGSMIHVQKWLGKETTIKRKIVDEKMVVECKMNNIVST RIYEKV |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于FABP9重组蛋白的虚构参考文献示例(注：文献信息为模拟生成，非真实存在)：
1. **文献名称**: "Expression and purification of recombinant FABP9 in Escherichia coli for functional studies"
**作者**: Zhang, Y. et al.
**摘要**: 该研究成功构建了大肠杆菌表达系统生产FABP9重组蛋白，优化了诱导表达条件并通过亲和层析纯化获得高纯度蛋白，为后续配体结合实验提供基础。
2. **文献名称**: "Structural insights into FABP9-ligand interactions using X-ray crystallography"
**作者**: Thompson, R.W. & Lee, S.K.
**摘要**: 通过X射线晶体学解析了FABP9重组蛋白的三维结构，揭示了其与脂肪酸配体的结合位点及构象变化机制，提示其在精子能量代谢中的潜在作用。
3. **文献名称**: "FABP9 knockout mice exhibit impaired spermatogenesis linked to lipid metabolism dysregulation"
**作者**: Gupta, A. et al.
**摘要**: 利用重组FABP9蛋白进行体外补偿实验，发现其缺失导致小鼠精子发育异常，证实FABP9通过调控睾丸特异性脂质转运影响生育能力。
*注：以上文献为模拟内容，实际研究中请通过PubMed或Web of Science等平台检索真实文献。*
Fatty Acid-Binding Protein 9 (FABP9), also known as testis-FABP (TFABP), is a member of the intracellular lipid-binding protein family. It is primarily expressed in the male reproductive system, particularly in germ cells during spermatogenesis. FABP9 is structurally characterized by a conserved β-barrel motif that facilitates the binding and transport of hydrophobic ligands, such as fatty acids, retinoic acid, and other lipid signaling molecules. With a molecular weight of approximately 14-15 kDa, it plays a critical role in regulating lipid metabolism, membrane biosynthesis, and energy homeostasis in developing sperm cells. Its expression is tightly associated with post-meiotic stages of spermatogenesis, suggesting a functional link to sperm maturation and acrosome formation.
Recombinant FABP9 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. This involves cloning the FABP9 gene into expression vectors, followed by affinity chromatography (e.g., His-tag purification) and refolding steps if required. The recombinant protein retains ligand-binding capacity, enabling studies on its interaction with lipids, structural dynamics, and cellular pathways. Researchers utilize it to investigate FABP9's role in male fertility, including its impact on sperm motility, membrane stability, and oxidative stress responses. Additionally, it serves as an antigen for antibody development and a tool to explore correlations between FABP9 dysregulation and infertility or testicular pathologies. Recent studies also highlight its potential as a biomarker for reproductive health or a target for contraceptive strategies.
×
