| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBP2 |
| Uniprot No | O00757 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-339aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMTDRSPFETDMLTLTRYVMEKGRQAKG TGELTQLLNSMLTAIKAISSAVRKAGLAHLYGIAGSVNVTGDEVKKLDVL SNSLVINMVQSSYSTCVLVSEENKDAIITAKEKRGKYVVCFDPLDGSSNI DCLASIGTIFAIYRKTSEDEPSEKDALQCGRNIVAAGYALYGSATLVALS TGQGVDLFMLDPALGEFVLVEKDVKIKKKGKIYSLNEGYAKYFDAATTEY VQKKKFPEDGSAPYGARYVGSMVADVHRTLVYGGIFLYPANQKSPKGKLR LLYECNPVAYIIEQAGGLATTGTQPVLDVKPEAIHQRVPLILGSPEDVQE YLTCVQKNQAGS |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **FBP2重组蛋白** 的3篇参考文献及其摘要概括：
1. **文献名称**：*"Recombinant human FBP2: expression, purification, and characterization of a key enzyme in gluconeogenesis"*
**作者**：Li Y, et al.
**摘要**：该研究报道了人源FBP2重组蛋白在大肠杆菌中的高效表达与纯化方法，通过His标签亲和层析获得高纯度蛋白，并验证其酶活性和热稳定性，为后续代谢疾病研究提供工具。
2. **文献名称**：*"Structural insights into the allosteric regulation of FBP2 via recombinant protein crystallography"*
**作者**：Zhang Q, et al.
**摘要**：利用重组表达的FBP2蛋白进行X射线晶体学分析，揭示了其变构调节位点的三维结构，阐明了ATP和AMP结合对酶活性的调控机制，为靶向FBP2的药物设计奠定基础。
3. **文献名称**：*"Functional analysis of FBP2 in cancer metabolism using recombinant protein-based assays"*
**作者**：Wang H, et al.
**摘要**：通过哺乳动物细胞表达系统制备功能性FBP2重组蛋白，结合体外代谢实验证明其在肿瘤细胞糖异生抑制和氧化应激调控中的双重作用，提示其作为癌症治疗靶点的潜力。
4. **文献名称**：*"Development of a novel FBP2 knockout model with recombinant adeno-associated virus delivery"*
**作者**：Chen L, et al.
**摘要**：构建基于重组腺相关病毒(rAAV)的FBP2基因敲除系统，结合重组蛋白功能回补实验，证实FBP2缺失导致线粒体能量代谢紊乱，为研究其在代谢综合征中的作用提供新方法。
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**注**：以上文献信息为模拟示例，实际文献需通过PubMed或Web of Science等平台检索确认。
Fructose-1.6-bisphosphatase 2 (FBP2), also known as muscle-type fructose-1.6-bisphosphatase, is a key metabolic enzyme involved in gluconeogenesis and energy homeostasis. It catalyzes the hydrolysis of fructose-1.6-bisphosphate to fructose-6-phosphate, a rate-limiting step that opposes glycolysis. Unlike its liver-specific isoform FBP1. FBP2 is predominantly expressed in muscle tissues and plays dual roles in regulating glucose metabolism and cellular processes beyond energy production.
The interest in recombinant FBP2 protein stems from its emerging biological significance in human diseases. Studies have linked FBP2 dysfunction to metabolic disorders, including type 2 diabetes and rare genetic diseases affecting glycogen storage. More recently, FBP2 has gained attention in cancer research, where it acts as a tumor suppressor in various malignancies by modulating metabolic reprogramming and apoptosis. Its downregulation in cancers like glioblastoma and leukemia has been associated with poor prognosis, sparking interest in FBP2-based therapeutic strategies.
Recombinant FBP2 production typically employs bacterial (E. coli) or mammalian expression systems, enabling researchers to obtain high-purity protein for structural and functional studies. This engineered protein has become crucial for investigating FBP2's non-catalytic roles in chromatin remodeling, cellular differentiation, and oxidative stress response. Pharmaceutical applications include drug screening for metabolic regulators and developing FBP2-targeted therapies. Current research focuses on understanding its tissue-specific regulation, post-translational modifications, and potential as a biomarker for metabolic syndromes or cancer progression. The development of recombinant FBP2 antibodies further supports diagnostic applications and mechanistic studies in disease models.
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