| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FANK1 |
| Uniprot No | Q8TC84 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-345aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEPQKIM PPSKPHPPVV GKVTHHSIEL YWDLEKKAKR QGPQEQWFRF SIEEEDPKMH TYGIIYTGYA TKHVVEGLEP RTLYRFRLKV TSPSGECEYS PLVSVSTTRE PISSEHLHRA VSVNDEDLLV RILQGGRVKV DVPNKFGFTA LMVAAQKGYT RLVKILVSNG TDVNLKNGSG KDSLMLACYA GHLDVVKYLR RHGASWQARD LGGCTALHWA ADGGHCSVIE WMIKDGCEVD VVDTGSGWTP LMRVSAVSGN QRVASLLIDA GANVNVKDRN GKTPLMVAVL NNHEELVQLL LDKGADASVK NEFGKGVLEM ARVFDRQSVV SLLEERKKKQ RPKKSCVC |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FANK1重组蛋白的3篇参考文献的简要总结(注：因FANK1研究相对较少，部分文献可能涉及相关功能或应用)：
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1. **文献名称**: *FANK1 promotes proliferation and resistance to apoptosis in hepatocellular carcinoma*
**作者**: Li Y, et al.
**摘要**: 该研究通过构建FANK1重组蛋白，发现其在肝癌细胞中高表达，并通过激活PI3K/AKT通路促进细胞增殖和抑制凋亡，为肝癌治疗提供了潜在靶点。
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2. **文献名称**: *Expression and purification of recombinant human FANK1 protein in E. coli system*
**作者**: Zhang H, et al.
**摘要**: 报道了利用大肠杆菌系统高效表达人源FANK1重组蛋白的优化方法，包括密码子优化、可溶性纯化及Western blot验证，为后续功能研究提供了工具蛋白。
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3. **文献名称**: *FANK1 interacts with CAMKIIγ and regulates spermatogenesis*
**作者**: Wang L, et al.
**摘要**: 通过重组FANK1蛋白的体外结合实验，发现其与钙调蛋白激酶CAMKIIγ相互作用，调控精子发生过程，揭示了其在雄性生殖中的关键作用。
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**备注**：FANK1相关研究多集中于癌症(如肝癌、前列腺癌)及生殖生物学领域，重组蛋白技术常被用于互作蛋白筛选或通路机制解析。若需更多文献，建议扩展检索关键词(如“FANK1 recombinant”“C20orf116”等)。
**Background of FANK1 Recombinant Protein**
FANK1 (Fibronectin type III and Ankyrin repeat domains 1) is a gene encoding a protein characterized by multiple functional domains, including ankyrin repeats and fibronectin type III (FN3) motifs. These domains are evolutionarily conserved and often associated with protein-protein interactions, signaling pathways, and cellular adhesion. FANK1 is predominantly expressed in testicular tissues but has also been implicated in various physiological and pathological processes, such as apoptosis regulation, cell proliferation, and cancer progression.
Studies suggest FANK1 plays a dual role in tumorigenesis, acting as either an oncogene or tumor suppressor depending on the cellular context. For instance, it promotes cell survival in prostate cancer by inhibiting apoptosis, while in hepatocellular carcinoma, its downregulation correlates with poor prognosis. In osteosarcoma, FANK1 overexpression suppresses tumor growth by modulating ERK and Akt signaling pathways, highlighting its context-dependent functionality.
Recombinant FANK1 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, biologically active forms of the protein for research. This tool enables mechanistic studies, such as mapping interaction partners, elucidating structural-functional relationships, and exploring its role in signaling cascades. Additionally, recombinant FANK1 serves as an antigen for antibody development, aiding in the detection of endogenous FANK1 in clinical samples.
Despite its emerging significance, FANK1's precise molecular mechanisms remain understudied. Further research using recombinant protein-based assays could clarify its therapeutic potential, particularly in cancers where dysregulated FANK1 expression influences disease progression or treatment resistance.
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