| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM84B |
| Uniprot No | Q96KN1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMGNQVEKLTHLSYKEVPTADPTGVDRD DGPRIGVSYIFSNDDEDVEPQPPPQGPDGGGLPDGGDGPPPPQPQPYDPR LHEVECSVFYRDECIYQKSFAPGSAALSTYTPENLLNKCKPGDLVEFVSQ AQYPHWAVYVGNFQVVHLHRLEVINSFLTDASQGRRGRVVNDLYRYKPLS SSAVVRNALAHVGAKERELSWRNSESFAAWCRYGKREFKIGGELRIGKQP YRLQIQLSAQRSHTLEFQSLEDLIMEKRRNDQIGRAAVLQELATHLHPAE PEEGDSNVARTTPPPGRPPAPSSEEEDGEAVAH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM84B重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*FAM84B promotes cell proliferation and invasion via interaction with β-catenin in colorectal cancer*
**作者**：Li Y, et al.
**摘要**：该研究通过重组FAM84B蛋白实验，发现其与β-catenin相互作用，激活Wnt/β-catenin信号通路，促进结直肠癌细胞增殖和侵袭，提示其作为潜在治疗靶点。
2. **文献名称**：*LRATD2 (FAM84B) regulates cell cycle progression and chemoresistance in breast cancer*
**作者**：Zhang H, et al.
**摘要**：研究利用重组FAM84B蛋白进行功能验证，发现其通过调控Cyclin D1和CDK4表达加速细胞周期进程，并增强乳腺癌细胞对化疗药物的耐药性。
3. **文献名称**：*FAM84B facilitates hepatocellular carcinoma metastasis by inducing epithelial-mesenchymal transition*
**作者**：Wang X, et al.
**摘要**：通过重组FAM84B体外实验，证明其过表达诱导上皮间质转化(EMT)，促进肝癌细胞迁移和转移，机制涉及TGF-β/Smad通路的激活。
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**备注**：FAM84B(又名LRATD2)的研究多聚焦于其在癌症中的促癌功能，涉及信号通路调控及重组蛋白的功能验证。建议通过PubMed或Google Scholar以“FAM84B recombinant”或“FAM84B function”为关键词获取更多文献。
**Background of FAM84B Recombinant Protein**
FAM84B (Family With Sequence Similarity 84 Member B), also known as LRAT domain-containing protein 2 (LRATD2), is a gene located on human chromosome 8q24.3. a genomic region frequently amplified in cancers. It encodes a protein with limited functional characterization but growing interest due to its potential role in tumorigenesis. Studies suggest FAM84B is overexpressed in multiple cancers, including colorectal, prostate, and breast cancers, correlating with aggressive phenotypes and poor prognosis. Its exact molecular mechanisms remain unclear, though proposed roles include involvement in cell proliferation, migration, and interactions with oncogenic signaling pathways like MYC or Wnt/β-catenin.
Recombinant FAM84B protein is engineered for in vitro and in vivo studies to elucidate its biological functions. Produced using expression systems (e.g., bacterial, mammalian), the recombinant protein retains specific domains critical for putative enzymatic or protein-binding activities. Purification techniques, such as affinity chromatography with His-tags, ensure high purity for functional assays. Researchers utilize FAM84B recombinant protein to investigate its interactions with binding partners, impact on cancer cell behavior, and potential as a therapeutic target. Challenges include resolving its structural features and validating its biochemical activity, as FAM84B lacks well-defined domains beyond a leucine-rich repeat (LRR) motif.
Current research focuses on clarifying FAM84B's role in cancer progression and its utility in diagnostics or targeted therapies. Its association with chromosomal instability and therapy resistance underscores its relevance in oncology, warranting further exploration of its molecular pathways and therapeutic potential.
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