| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM50A |
| Uniprot No | Q14320 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 150-339aa |
| 氨基酸序列 | TTKKRKLGKNPDVDTSFLPDRDREEEENRLREELRQEWEAKQEKIKSEEI EITFSYWDGSGHRRTVKMRKGNTMQQFLQKALEILRKDFSELRSAGVEQL MYIKEDLIIPHHHSFYDFIVTKARGKSGPLFNFDVHDDVRLLSDATVEKD ESHAGKVVLRSWYEKNKHIFPASRWEPYDPEKKWDKYTIR |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"FAM50A regulates mitotic progression by interacting with PLK1"**
*Authors: Li X, et al.*
摘要：研究发现FAM50A重组蛋白通过与有丝分裂关键激酶PLK1相互作用，调控细胞周期G2/M期转换，敲低FAM50A导致染色体排列异常和胞质分裂失败，提示其在基因组稳定性中的重要作用。
2. **"Structural insights into FAM50A-DNA interaction and implications for AML pathogenesis"**
*Authors: Wang Y, et al.*
摘要：通过X射线晶体学解析FAM50A重组蛋白的DNA结合结构域，揭示其与特定基因启动子结合的分子机制，并发现该蛋白在急性髓系白血病(AML)样本中异常高表达，可能参与表观遗传调控。
3. **"FAM50A functions as a tumor suppressor via Hippo signaling in hepatocellular carcinoma"**
*Authors: Tanaka R, et al.*
摘要：证明FAM50A重组蛋白通过激活Hippo通路抑制肝癌细胞增殖，临床数据分析显示FAM50A低表达与患者预后不良相关，其过表达显著降低移植瘤模型中肿瘤生长速度。
4. **"Proteomic characterization of FAM50A-interacting partners in neuronal development"**
*Authors: Gupta S, et al.*
摘要：利用重组FAM50A蛋白进行免疫共沉淀-质谱分析，鉴定出23个新型互作蛋白，包括微管相关蛋白MAP1B和突触形成因子NEUROD1.提示该蛋白在神经元迁移和轴突导向中的潜在作用。
(注：上述文献为模拟示例，实际研究需查阅具体数据库)
FAM50A (Family with sequence similarity 50 member A), also known as XAP5 or XAP5-like, is a nuclear protein encoded by the FAM50A gene located on the X chromosome (Xq28). It belongs to the conserved FAM50 protein family and is implicated in diverse cellular processes, including DNA damage response, cell cycle regulation, and transcriptional control. Structurally, FAM50A contains a zinc finger-like domain and nuclear localization signals, suggesting roles in DNA binding or protein interactions. Studies indicate its interaction with ATR kinase, a key regulator of DNA replication stress response, linking it to genome stability maintenance. Dysregulation of FAM50A has been associated with cancers (e.g., leukemia, breast cancer) and neurodevelopmental disorders, particularly X-linked intellectual disability (XLID), where truncating mutations disrupt its function.
Recombinant FAM50A protein is engineered using expression systems like *E. coli* or mammalian cells, enabling functional studies. Its production typically involves cloning the coding sequence into expression vectors, followed by purification via affinity tags (e.g., His-tag). The recombinant protein serves as a critical tool for investigating FAM50A’s molecular mechanisms, including its role in ATR-mediated signaling, cell cycle checkpoints, and chromatin remodeling. Researchers also utilize it to map protein interaction networks, screen for binding partners, and develop antibodies for diagnostic or therapeutic exploration. Despite progress, FAM50A’s precise molecular functions remain partially characterized, necessitating further research to clarify its contributions to disease pathways and potential as a therapeutic target.
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