| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM107B |
| Uniprot No | Q9H098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-131aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAEPDYI EDDNPELIRP QKLINPVKTS RNHQDLHREL LMNQKRGLAP QNKPELQKVM EKRKRDQVIK QKEEEAQKKK SDLEIELLKR QQKLEQLELE KQKLQEEQEN APEFVKVKGN LRRTGQEVAQ AQES |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM107B重组蛋白的3篇代表性文献的简要总结(注：文献为虚拟示例，实际研究请通过学术数据库检索)：
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1. **文献名称**: "Expression and functional characterization of recombinant FAM107B protein in cancer cell lines"
**作者**: Li X, et al. (2018)
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组FAM107B蛋白，发现其在肺癌组织中表达下调。体外实验表明，过表达FAM107B可抑制癌细胞迁移，提示其可能通过调控细胞骨架重组发挥抑癌作用。
2. **文献名称**: "Structural insights into FAM107B protein by X-ray crystallography and its interaction with DNA"
**作者**: Wang Y, et al. (2021)
**摘要**: 通过X射线晶体学解析了重组FAM107B蛋白的三维结构，发现其含有一个保守的DNA结合结构域。实验证实FAM107B可与特定DNA序列结合，暗示其在表观遗传调控或转录调控中的潜在功能。
3. **文献名称**: "FAM107B recombinant protein suppresses hepatocellular carcinoma progression via modulating apoptosis pathways"
**作者**: Chen L, et al. (2022)
**摘要**: 在肝细胞癌模型中，重组FAM107B蛋白显著诱导癌细胞凋亡，并抑制裸鼠移植瘤生长。机制研究表明，其通过激活Caspase-3和抑制Bcl-2表达发挥作用，提示其作为治疗靶点的潜力。
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**注意**：以上文献为模拟内容，实际研究中FAM107B的功能尚未完全明确，建议通过PubMed、Web of Science等平台以“FAM107B recombinant protein”为关键词检索最新进展。部分研究可能侧重其与神经发育或肿瘤微环境的关联。
FAM107B (Family with sequence similarity 107 member B) is a protein-coding gene located on human chromosome 10q25.2. The encoded protein, also known as DRR1 (down-regulated in renal cell carcinoma 1), is a conserved, ubiquitously expressed molecule with roles in cellular processes such as cytoskeletal regulation, signal transduction, and transcriptional modulation. Structurally, FAM107B contains a coiled-coil domain and nuclear localization signals, suggesting involvement in protein-protein interactions and nuclear functions. While its exact molecular mechanisms remain under investigation, FAM107B has been implicated in tumor suppression, particularly in renal, gastric, and breast cancers, where its expression is often reduced due to epigenetic silencing or chromosomal deletions.
Studies link FAM107B to cancer progression through pathways involving cell adhesion, migration, and apoptosis. It interacts with cytoskeletal components like actin and microtubules, potentially influencing cell motility and metastasis. Beyond oncology, emerging evidence associates FAM107B with neurodevelopment, as it regulates neurite outgrowth and neuronal differentiation. Animal models demonstrate its importance in brain development, with knockout mice showing behavioral abnormalities. The recombinant FAM107B protein, typically produced in E. coli or mammalian expression systems with affinity tags (e.g., His-tag), enables functional studies, antibody production, and structural analyses. Its recombinant form has become crucial for elucidating interaction networks, post-translational modifications, and therapeutic targeting strategies. Current research focuses on validating its diagnostic/prognostic potential in cancers and exploring its role in neurological disorders, highlighting its dual significance in cellular homeostasis and disease pathogenesis.
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