| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FADD |
| Uniprot No | Q13158 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS |
| 预测分子量 | 30.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis"**
*作者：Chinnaiyan AM, et al.*
摘要：首次克隆并鉴定了FADD蛋白，证实其通过死亡结构域与Fas受体结合，激活caspase级联反应，调控细胞凋亡通路。
2. **"Targeted disruption of the FADD gene causes early embryonic lethality and defects in lymphocyte apoptosis"**
*作者：Yeh WC, et al.*
摘要：通过基因敲除实验发现FADD缺失导致胚胎死亡及T细胞凋亡异常，证明FADD在发育和免疫系统中的必需作用。
3. **"Recombinant FADD protein enhances TRAIL-induced apoptosis through promoting caspase-8 activation"**
*作者：Zhang L, et al.*
摘要：研究重组FADD蛋白在TRAIL信号通路中的作用，证明其通过促进caspase-8活化显著增强肿瘤细胞凋亡敏感性。
4. **"Expression and purification of functional human FADD in E. coli for structural studies"**
*作者：Wang X, et al.*
摘要：优化了FADD重组蛋白在大肠杆菌中的可溶性表达与纯化工艺，为基于结构的药物设计提供高纯度样本。
FADD (Fas-associated protein with death domain) is a pivotal adaptor protein involved in apoptotic signaling pathways and immune regulation. Discovered in the mid-1990s, it plays a central role in transmitting death signals from cell surface death receptors, such as Fas (CD95) and TNF receptor superfamily members, to intracellular caspase cascades. Structurally, FADD contains two conserved domains: an N-terminal death effector domain (DED) that interacts with initiator caspases like caspase-8/10. and a C-terminal death domain (DD) responsible for binding activated death receptors. This bridging function enables FADD to assemble the death-inducing signaling complex (DISC), triggering caspase activation and programmed cell death.
Recombinant FADD protein refers to the engineered version produced using expression systems like *E. coli* or mammalian cells. Its production involves cloning the FADD gene into expression vectors, followed by purification via affinity chromatography. Recombinant technology ensures high purity, specificity, and controlled post-translational modifications, making it invaluable for mechanistic studies. Researchers employ recombinant FADD to investigate apoptosis regulation, necroptosis crosstalk, and immune signaling dynamics in vitro. It also serves as a tool for screening therapeutic compounds targeting cell death pathways in cancer, autoimmune diseases, and neurodegenerative disorders. Recent studies highlight its involvement beyond apoptosis, including inflammasome modulation and T-cell receptor signaling, expanding its biomedical relevance. The availability of recombinant FADD continues to advance our understanding of cell fate decisions and therapeutic interventions in death receptor-mediated pathologies.
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